Differentiating the functional role of hypoxia-inducible factor (HIF)-1alpha and HIF-2alpha (EPAS-1) by the use of RNA interference: erythropoietin is a HIF-2alpha target gene in Hep3B and Kelly cells.

@article{Warnecke2004DifferentiatingTF,
  title={Differentiating the functional role of hypoxia-inducible factor (HIF)-1alpha and HIF-2alpha (EPAS-1) by the use of RNA interference: erythropoietin is a HIF-2alpha target gene in Hep3B and Kelly cells.},
  author={Christina Warnecke and Zaneta Zaborowska and Jens Kurreck and Volker A. Erdmann and Ulrich A Frei and Michael Sean Wiesener and K E Eckardt},
  journal={FASEB journal : official publication of the Federation of American Societies for Experimental Biology},
  year={2004},
  volume={18 12},
  pages={1462-4}
}
Activation of the hypoxia-inducible factor alpha-subunits, HIF-1alpha and HIF-2alpha, seems to be subject to similar regulatory mechanisms, and transgene approaches suggested partial functional redundancy. Here, we used RNA interference to determine the contribution of HIF-1alpha vs. HIF-2alpha to the hypoxic gene induction. Surprisingly, most genes tested… CONTINUE READING