Differentiating passive from transporter-mediated uptake by PepT1: a comparison and evaluation of four methods.

@article{Scow2011DifferentiatingPF,
  title={Differentiating passive from transporter-mediated uptake by PepT1: a comparison and evaluation of four methods.},
  author={Jeffrey S. Scow and Srivats Madhavan and Rizwan M. Chaudhry and Ye Zheng and Judith A. Duenes and Michael G Sarr},
  journal={The Journal of surgical research},
  year={2011},
  volume={170 1},
  pages={
          17-23
        }
}
BACKGROUND To quantify transmembrane transport of dipeptides by PepT1, passive uptake (non-PepT1 mediated) must be subtracted from total (measured) uptake. Three methods have been described to estimate passive uptake: perform experiments at cold temperatures, inhibit target dipeptide uptake with a greater concentration of a second dipeptide, or use modified Michaelis-Menten kinetics. We hypothesized that performing uptake experiments at pH 8.0 would estimate passive uptake accurately, because… CONTINUE READING
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