Differentiating Dracunculus medinensis from D. insignis, by the sequence analysis of the 18S rRNA gene

@article{Bimi2005DifferentiatingDM,
  title={Differentiating Dracunculus medinensis from D. insignis, by the sequence analysis of the 18S rRNA gene},
  author={Langbong Bimi and Amanda R. Freeman and Mark L. Eberhard and Ernesto Ruiz-Tiben and Norman J. Pieniązek},
  journal={Annals of Tropical Medicine \& Parasitology},
  year={2005},
  volume={99},
  pages={511 - 517}
}
Abstract This study, undertaken as a component of the global Dracunculiasis Eradication Program (DEP), was designed to provide molecular tools to distinguish Dracunculus medinensis, the nematode causing human dracunculiasis, from other tissue-dwelling nematodes, including other Dracunculus species that infect humans and other animals. DNA was extracted from D. medinensis and from a closely related species that infects North American carnivores, D. insignis, so that the genes coding for the… 

PCR-Based Detection of Angiostrongylus cantonensis in Tissue and Mucus Secretions from Molluscan Hosts

TLDR
This is the first use of a PCR-based method to confirm the presence of A. cantonensis in mollusks collected in the environment, and shows that the two methods were equally sensitive and specific for this application.

PCR-Based Detection of Angiostrongylus cantonensis in Tissue and Mucus Secretions from Molluscan Hosts (cid:1)

TLDR
To the authors' knowledge, this is the first use of a PCR-based method to detect the presence of A. cantonensis in mollusks collected in the environment and the two methods were equally sensitive and specific for this application.

Dracunculus Species in Meso-mammals from Georgia, United States, and Implications for the Guinea Worm Eradication Program in Chad, Africa.

TLDR
Investigation of the prevalence of infections with Dracunculus species among wildlife at a single site (Di-Lane plantation) in the southeastern United States, evaluate the genetic diversity of parasites at this site, and investigate potential paratenic hosts that may be involved in transmission increases knowledge of the potential role of aquatic animals in the transmission of Drac unculus species and informs on potential intervention strategies that might be applied to the eradication of Guinea worm in Africa.

Species identification of North American guinea worms (Nematoda: Dracunculus) with DNA barcoding

TLDR
DNA barcoding is used to differentiate between these two North American dracunculoids, showing that D. insignis is a ‘true’ generalist, showing little sequence divergence regardless of host association, although these studies did validate its occurrence in a new host — the otter.

Population genomic evidence that human and animal infections in Africa come from the same populations of Dracunculus medinensis

Background Guinea worm – Dracunculus medinensis – was historically one of the major parasites of humans and has been known since antiquity. Now, Guinea worm is on the brink of eradication, as efforts

The wild world of Guinea Worms: A review of the genus Dracunculus in wildlife

Human infection with an unknown species of Dracunculus in Vietnam.

  • P. ThachH. V. van Doorn Le Van Duyet
  • Medicine, Biology
    International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases
  • 2021

First report of Dracunculus insignis in two naturally infected cats from the northeastern USA

TLDR
These two cases appear to be the first published reports of dracunculiasis in domestic cats in the USA, and based on the findings from case 1, D insignis may be the species associated with both infections.

References

SHOWING 1-10 OF 25 REFERENCES

Redescription of Dracunculus globocephalus Mackin, 1927 (Nematoda: Dracunculidae), a parasite of the snapping turtle, Chelydra serpentina.

TLDR
This species markedly differs from all other species of Dracunculus in having the spicules greatly unequal in size and shape, in the absence of a gubernaculum, and in the disposition of male caudal papillae.

OBSERVATIONS ON THE SEASONAL PREVALENCE, PATHOLOGY AND TRANSMISSION OF Dracunculus insignis (NEMATODA: DRACUNCULOIDEA) IN THE RACCOON (Procyon lotor (L.) IN ONTARIO1

TLDR
Crayfish, fishes and frogs were given infective third-stage larvae of D. insignis to test their suitability as paratenic hosts and found they had increased in size and were highly infective to raccoons.

A molecular evolutionary framework for the phylum Nematoda

TLDR
It is suggested that animal parasitism arose independently at least four times, and plant parasitism three times, which indicates that convergent morphological evolution may be extensive and that present higher-level classification of the Nematoda will need revision.

Dracunculus and dracunculiasis.

Nematode transmission patterns.

The transmission of nematode parasites of vertebrates is reviewed with special reference to the phenomena of monoxeny, heteroxeny, paratenesis, and precocity. Monoxeny is divided into 2 types.

Human case of dracunculiasis in Japan.

TLDR
The worm's morphology and that of the embryos found in its uterus revealed that the parasite was Dracunculus medinensis, the first human case of dracunculiasis acquired in Japan.

Quartet Puzzling: A Quartet Maximum-Likelihood Method for Reconstructing Tree Topologies

TLDR
A versatile method, quartet puzzling, is introduced to reconstruct the topology (branching pattern) of a phylogenetic tree based on DNA or amino acid sequence data and outperforms neighbor joining in some cases with high transition/transversion bias.

TreeView: an application to display phylogenetic trees on personal computers

  • R. Page
  • Computer Science
    Comput. Appl. Biosci.
  • 1996
TreeView is a simple, easy to use phylogenetic tree viewing utility that runs under both MacOS (on Apple Macintosh computers) and under Microsoft Windows on Intel based computers, the two most common

CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice.

TLDR
The sensitivity of the commonly used progressive multiple sequence alignment method has been greatly improved and modifications are incorporated into a new program, CLUSTAL W, which is freely available.