Differential translation tunes uneven production of operon-encoded proteins.

Abstract

Clustering of functionally related genes in operons allows for coregulated gene expression in prokaryotes. This is advantageous when equal amounts of gene products are required. Production of protein complexes with an uneven stoichiometry, however, requires tuning mechanisms to generate subunits in appropriate relative quantities. Using comparative genomic analysis, we show that differential translation is a key determinant of modulated expression of genes clustered in operons and that codon bias generally is the best in silico indicator of unequal protein production. Variable ribosome density profiles of polycistronic transcripts correlate strongly with differential translation patterns. In addition, we provide experimental evidence that de novo initiation of translation can occur at intercistronic sites, allowing for differential translation of any gene irrespective of its position on a polycistronic messenger. Thus, modulation of translation efficiency appears to be a universal mode of control in bacteria and archaea that allows for differential production of operon-encoded proteins.

DOI: 10.1016/j.celrep.2013.07.049
0502014201520162017
Citations per Year

117 Citations

Semantic Scholar estimates that this publication has 117 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Quax2013DifferentialTT, title={Differential translation tunes uneven production of operon-encoded proteins.}, author={Tessa E F Quax and Yuri I Wolf and Jasper J Koehorst and Omri Wurtzel and Richard van der Oost and Wenqi Ran and Fabian Blombach and Kira S Makarova and Stan J J Brouns and Anthony C Forster and E Gerhart H Wagner and Rotem Sorek and Eugene V Koonin and John van der Oost}, journal={Cell reports}, year={2013}, volume={4 5}, pages={938-44} }