Differential suppression of persistent insect specific viruses in trans-infected wMel and wMelPop-CLA Aedes-derived mosquito lines

  title={Differential suppression of persistent insect specific viruses in trans-infected wMel and wMelPop-CLA Aedes-derived mosquito lines},
  author={Breeanna J. McLean and Kimberley R. Dainty and Heather A Flores and S. O'Neill},
  pages={141 - 145}
Wolbachia suppresses the replication of +ssRNA viruses such as dengue and Zika viruses in Aedes aegypti mosquitoes. However, the range of viruses affected by this endosymbiont is yet to be explored. Recently, novel insect-specific viruses (ISVs) have been described from numerous mosquito species and mosquito-derived cell lines. Cell-fusing agent virus (Flaviviridae) and Phasi Charoen-like virus (Bunyaviridae) persistently infect the Ae. aegypti cell line Aag2 which has been used for… Expand
Uncovering the Worldwide Diversity and Evolution of the Virome of the Mosquitoes Aedes aegypti and Aedes albopictus
Aedes aegypti, the yellow fever mosquito, and Aedes albopictus, the Asian tiger mosquito, are the most significant vectors of dengue, Zika, and Chikungunya viruses globally. Studies examining hostExpand
Using Wolbachia to Eliminate Dengue: Will the Virus Fight Back?
While there are hurdles to dengue viruses developing resistance to Wolbachia, it is suggested that long-term surveillance for resistant viruses should be an integral component of Wolbachian-introgression biocontrol programs. Expand
Wolbachia modulates prevalence and viral load of Culex pipiens densoviruses in natural populations
Analyzing the interactions between a widely prevalent ISV, the Culex pipiens densovirus (CpDV, Densovirinae), and Wolbachia in northern Tunisian C. pipiens populations provided good evidence for an ecological influence of WolbachIA on anISV, CpDVs, in natural populations and highlighted the importance of integrating Wolbachias in the understanding of ISV ecology in nature. Expand
Symbiotic Interactions Between Mosquitoes and Mosquito Viruses
Mosquitoes not only transmit human and veterinary pathogens called arboviruses (arthropod-borne viruses) but also harbor mosquito-associated insect-specific viruses (mosquito viruses) that cannotExpand
A Novel Anphevirus in Aedes albopictus Mosquitoes Is Distributed Worldwide and Interacts with the Host RNA Interference Pathway
The Asian tiger mosquito Aedes albopictus is a competent vector for several human arboviruses including dengue, chikungunya and Zika viruses. Mosquitoes also harbor insect-specific viruses (ISVs)Expand
Growth and Maintenance of Wolbachia in Insect Cell Lines
Simple Summary Wolbachia is an intracellular bacterium that occurs in arthropods and in filarial worms. First described nearly a century ago in the reproductive tissues of Culex pipiens mosquitoes,Expand
Exploiting insect-specific viruses as a novel strategy to control vector-borne disease.
A greater understanding of ISVs' basic biology is required, including their species range, ability to be maintained and transmitted in native and non-native vector hosts, and the effect of infection on a range of pathogens. Expand
Zika virus infection and temperature significantly impact Aedes albopictus gut microbiota
It is demonstrated that both temperature and ZIKV infection modulate the midgut microbial profile of Ae. Expand


Effect of Wolbachia on Replication of West Nile Virus in a Mosquito Cell Line and Adult Mosquitoes
The results suggest that despite the enhancement of viral genomic RNA replication in the Wolbachia-infected cell line the production of secreted virus was significantly inhibited, the antiviral effect in intrathoracically infected mosquitoes depends on the strain of Wol Bachia, and replication of the virus in orally fed mosquitoes was completely inhibited in wMelPop strain of Wbachia. Expand
Wolbachia suppresses cell fusing agent virus in mosquito cells.
Investigation of the effect of Wolbachia, a widespread endosymbiont of many insect species, that is known to block replication of several pathogenic flaviviruses, on CFAV suggests that WolbachIA is also able to suppress an ISF. Expand
Wolbachia restricts insect-specific flavivirus infection in Aedes aegypti cells
It is shown that transinfection of the Drosophila-derived wMelPop Wolbachia strain into Aedes aegypti-derived cells resulted in inhibition and even clearance of the persistent cell-fusing agent flavivirus infection in these cells, which broadens the antiviral activity of Wol Bachia from acute infections to persistent infections and from arboviruses to mosquito-specific viruses. Expand
Aedes Anphevirus: an Insect-Specific Virus Distributed Worldwide in Aedes aegypti Mosquitoes That Has Complex Interplays with Wolbachia and Dengue Virus Infection in Cells
Aedes anphevirus (AeAV), a negative-sense RNA virus from the order Mononegavirales, is characterized and Wolbachia enhances the replication of AeAV and modestly reduces dengue virus replication in a cell line model. Expand
Wolbachia wStri Blocks Zika Virus Growth at Two Independent Stages of Viral Replication
ABSTRACT Mosquito-transmitted viruses are spread globally and present a great risk to human health. Among the many approaches investigated to limit the diseases caused by these viruses are attemptsExpand
Limited Dengue Virus Replication in Field-Collected Aedes aegypti Mosquitoes Infected with Wolbachia
The results indicate that virus-blocking is likely to persist in Wolbachia-infected mosquitoes after their release and establishment in wild populations, suggesting that Wolbachian biocontrol may be a successful strategy for reducing dengue transmission in the field. Expand
Aedes anphevirus (AeAV): an insect-specific virus distributed worldwide in Aedes aegypti mosquitoes that has complex interplays with Wolbachia and dengue virus infection in cells
Wolbachia enhances the replication of AeAV and modestly reduces dengue virus replication in a cell line model, which improves understanding of the diversity and evolution of the virome of Ae. Expand
Wolbachia Induces Density-Dependent Inhibition to Dengue Virus in Mosquito Cells
There is a strong negative linear correlation between the genome copy of Wolbachia and d Dengue virus with a dengue infection completely removed when Wolbacha density reaches a certain level, which will aid in understanding the mechanism of Wol Bachia-mediated pathogen interference and developing novel methods to block disease transmission by mosquitoes carrying native Wolbachian infections. Expand
A novel insect-specific flavivirus replicates only in Aedes-derived cells and persists at high prevalence in wild Aedes vigilax populations in Sydney, Australia.
Investigation of the diversity and prevalence of ISFs in Australian mosquitoes, samples from various regions were screened for flaviviruses by ELISA and RT-PCR and revealed PaRV had persisted in A. vigilax populations in Sydney, with 88% of pools positive. Expand
The wMel strain of Wolbachia Reduces Transmission of Zika virus by Aedes aegypti
Zika virus (ZIKV) is causing an explosive outbreak of febrile disease in the Americas. There are no effective antiviral therapies or licensed vaccines for this virus, and mosquito control strategiesExpand