Differential stiffness and lipid mobility in the leaflets of purple membranes.

  title={Differential stiffness and lipid mobility in the leaflets of purple membranes.},
  author={Kislon Voϊtchovsky and Sonia Antoranz Contera and Miya Kamihira and Anthony Watts and John F. Ryan},
  journal={Biophysical journal},
  volume={90 6},
Purple membranes (PM) are two-dimensional crystals formed by bacteriorhodopsin and a variety of lipids. The lipid composition and density in the cytoplasmic (CP) leaflet differ from those of the extracellular (EC) leaflet. A new way of differentiating the two sides of such asymmetric membranes using the phase signal in alternate contact atomic force microscopy is presented. This method does not require molecular resolution and is applied to study the stiffness and intertrimer lipid mobility in… CONTINUE READING


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