Differential staining of neurons in the human retina with antibodies to protein kinase C isozymes.

@article{Kolb1993DifferentialSO,
  title={Differential staining of neurons in the human retina with antibodies to protein kinase C isozymes.},
  author={Helga Kolb and Limin Zhang and Laura Dekorver},
  journal={Visual neuroscience},
  year={1993},
  volume={10 2},
  pages={341-51}
}
Monoclonal antibodies to the three isozymes of protein kinase C (PKC) (alpha, beta, and gamma) were applied to postmortem human retina. Immunostaining was done on wholemount, or cryostat-sectioned retina, and visualized after ABC/DAB procedures by light (LM) and electron (EM) microscopy. The PCK-alpha antibody stained rod bipolar cells throughout the retina. EM analysis confirmed they were PKC-alpha-immunoreactive (IR) on their characteristic dendritic and axonal synaptology. Putative blue cone… CONTINUE READING
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