Differential solubilization of rabbit liver plasma membrane gamma-glutamyltransferase by proteases and detergents: effect of phenobarbital treatment.


In an attempt to understand the mechanism of gamma-glutamyltransferase transfer from the liver to the plasma, potassium chloride, sodium dodecyl sulfate and proteases (papain and bromelain) were used to solubilize rabbit liver plasma membrane gamma-glutamyltransferase. Potassium chloride solutions solubilized 10-30% of membrane proteins but only 1-3% of… (More)


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