Differential solubilization of lipids along with membrane proteins by different classes of detergents.

@article{Banerjee1995DifferentialSO,
  title={Differential solubilization of lipids along with membrane proteins by different classes of detergents.},
  author={Papia Banerjee and Joon Bum Joo and J T Buse and G. E. Dawson},
  journal={Chemistry and physics of lipids},
  year={1995},
  volume={77 1},
  pages={65-78}
}
Membrane proteins are typically extracted by detergent concentrations of 0.5-2.0%, using detergent/protein ratios of 1:1 to 3:1. We have compared the ability of 14 different detergents from seven different structural and ionic classes, at a concentration of 2.0% and a detergent/protein ratio of 2:1, to extract an integral membrane protein (the serotonin 5-HT1A receptor) in active form and have observed profound differences in both lipids and proteins. All extracts were freed from detergents and… CONTINUE READING
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