Differential serum dependence of cultured osteoclastic and osteoblastic bone cells

Sequential collagenase digestion of mice calvariae provides populations of bone cells that express either osteoclastic (OC) or osteoblastic (OB) activities after growth for 6 days in similar culture conditions consisting of minimal essential medium supplemented with 10% fetal calf serum (FCS). The OC characteristics (acid phosphatase activity and… CONTINUE READING