Differential sensitivity of pneumolysin-induced channels to gating by divalent cations

@article{Korchev1992DifferentialSO,
  title={Differential sensitivity of pneumolysin-induced channels to gating by divalent cations},
  author={Yuri E. Korchev and Christina Bashford and Charles A. Pasternak},
  journal={The Journal of Membrane Biology},
  year={1992},
  volume={127},
  pages={195-203}
}
The induction of channels across planar lipid bilayers by purified, recombinant pneumolysin (a hemolytic protein from Streptococcus pneumoniae) has been studied by measuring increases in electrical conductivity. Pneumolysin-induced channels exhibit a wide range of single channel conductances (<50 pS to >1 nS at 0.1 m KCl). Channels can be categorized on the basis of their K+:C− selectivity: the smallest channels are strongly cation selective, with t+ (the cation transference number) approaching… CONTINUE READING