Differential screening of murine ascites cDNA libraries by means of in vitro transcripts of cell-cycle-phase-specific cDNA and digital image processing.

@article{Lu1990DifferentialSO,
  title={Differential screening of murine ascites cDNA libraries by means of in vitro transcripts of cell-cycle-phase-specific cDNA and digital image processing.},
  author={X. Allan Lu and J Dengler and Karsten Rothbarth and D. Schmaljohann J. Oswald B. J\orgensen M. Nitschke D. Werner},
  journal={Gene},
  year={1990},
  volume={86 2},
  pages={185-92}
}
Cell-cycle-phase-specific cDNA libraries were prepared in the lambda gt10 vector and in the in vitro transcription vector, pBluescript. Plaques of the cDNA libraries prepared in the lambda gt10 vector were differentially screened with (a) in vitro transcripts of the cell-cycle-phase-specific cDNAs cloned in the transcription vector and (b) with first-strand cDNA of mRNA from phase-synchronous cells. The results suggest that first-strand cDNA can be replaced, at least in prescreening experiments… CONTINUE READING