Differential screening and mass mapping of proteins from premalignant and cancer cell lines using nonporous reversed-phase HPLC coupled with mass spectrometric analysis.

@article{Chong2001DifferentialSA,
  title={Differential screening and mass mapping of proteins from premalignant and cancer cell lines using nonporous reversed-phase HPLC coupled with mass spectrometric analysis.},
  author={B E Chong and Rick L Hamler and David M. Lubman and Stephen P Ethier and Allen J. Rosenspire and F. Roy Miller},
  journal={Analytical chemistry},
  year={2001},
  volume={73 6},
  pages={1219-27}
}
Nonporous (NPS) RP-HPLC has been used to rapidly separate proteins from whole cell lysates of human breast cell lines. The nonporous separation involves the use of hard-sphere silica beads of 1.5-microm diameter coated with C18, which can be used to separate proteins ranging from 5 to 90 kDa. Using only 30-40 microg of total protein, the protein molecular weights are detectable on-line using an ESI-oaTOF MS. Of hundreds of proteins detected in this mass range, approxinately 75-80 are more… CONTINUE READING
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