Differential roles of archaeal box H/ACA proteins in guide RNA-dependent and independent pseudouridine formation.

@article{Gurha2007DifferentialRO,
  title={Differential roles of archaeal box H/ACA proteins in guide RNA-dependent and independent pseudouridine formation.},
  author={Priyatansh Gurha and Archi Joardar and Priyasri Chaurasia and Ramesh Gupta},
  journal={RNA biology},
  year={2007},
  volume={4 2},
  pages={101-9}
}
RNA-guided pseudouridine (Psi) synthesis in Archaea and Eukarya requires a four-protein one-RNA containing box H/ACA ribonucleoprotein (RNP) complex. The proteins in the archaeal RNP are aCbf5, aNop10, aGar1 and L7Ae. Pyrococcus aCbf5-aNop10 is suggested to be the minimal catalytic core in this synthesis and the activity is enhanced by L7Ae and aGar1. The protein aCbf5 is homologous to eukaryal Cbf5 (dyskerin, NAP57) as well as to bacterial TruB and eukaryal Pus4; the last two produce YPsi55 in… CONTINUE READING