Differential response of human basophil activation markers: a multi-parameter flow cytometry approach

@article{Chirumbolo2008DifferentialRO,
  title={Differential response of human basophil activation markers: a multi-parameter flow cytometry approach},
  author={Salvatore Chirumbolo and Antonio Vella and Riccardo Ortolani and Marzia de Gironcoli and Pietro Solero and Giuseppe Tridente and Paolo Bellavite},
  journal={Clinical and Molecular Allergy : CMA},
  year={2008},
  volume={6},
  pages={12 - 12}
}
BackgroundBasophils are circulating cells involved in hypersensitivity reactions and allergy but many aspects of their activation, including the sensitivity to external triggering factors and the molecular aspects of cell responses, are still to be focused. In this context, polychromatic flow cytometry (PFC) is a proper tool to investigate basophil function, as it allows to distinguish the expression of several membrane markers upon activation in multiple experimental conditions.MethodsCell… 
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CCR3 as a single selection marker compared to CD123/HLADR to isolate basophils in flow cytometry: Some comments
TLDR
This work suggested the use of eotaxin receptor CCR3, also known as CD193, as a single selection marker to separate basophils from other leukocytes in flow cytometry, though with some controversial issue.
The expression of CD123 can decrease with basophil activation: implications for the gating strategy of the basophil activation test
TLDR
The use of CD203c before gating on CD123+/HLA-DR− cells or in isolation ensures the identification of the entire basophils population and accurate assessment of basophil activation, with important diagnostic implications.
Commentary: The Expression of CD123 Can Decrease with Basophil Activation: Implications for the Gating Strategy of the Basophil Activation Test
TLDR
It is shown that, during cellular activation, the expression of membrane CD123, the alpha subunit of the interleukin-3 (IL-3) receptor, is downregulated, leading to a possible loss in the electronic capture of basophils and hence a false negative outcome of the activation test.
Stimulus-specific regulation of CD63 and CD203c membrane expression in human basophils by the flavonoid quercetin.
CD164 as a Basophil Activation Marker.
TLDR
Although CD164 upregulation as a marker of basophil activation is a promising and powerful diagnostic tool, it still requires a lot of both basic research and comparative studies with older and well known markers, in order to select the best of them.
Analyzing histamine release by flow cytometry (HistaFlow): a novel instrument to study the degranulation patterns of basophils.
Monitoring of CD63% in basophil activation test and suggested new parameters for allergy diagnosis
TLDR
The relatively high frequency of involvement of this marker in allergy cellular tests necessitates a review of CD63 performance in BATs, and a closer look at how this molecule is expressed as a result of immunoglobulin (Ig) E-mediated activation has to be addressed.
Basophil Activation Test for Chronic Urticaria
TLDR
The authors identified the need to add the CD63 marker to the basophil activation test (BAT), as CD203c expression is dependent on primed basophils and that CD63 should be reported as a fundamental marker to assess basophIL activation.
Basophil Activation Antigens: Molecular Mechanisms and Clinical Implications
TLDR
A summary on activation-linked cell surface and cytoplasmic antigens in basophils, with special reference to poten- tial mechanisms underlying re-translocation or over-expression in activated cells, relevant signalling pathways, and clini- cal implications is presented.
Are Basophils and T helper 2 Cells Implicated in Mastocytosis
TLDR
The results argue against a possible role of basophils and Th2 cells in the episodes of mediator release observed in patients with mastocytosis.
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