Differential requirements for the O-linked branching enzyme core 2 beta1-6-N-glucosaminyltransferase in biosynthesis of ligands for E-selectin and P-selectin.

@article{Snapp2001DifferentialRF,
  title={Differential requirements for the O-linked branching enzyme core 2 beta1-6-N-glucosaminyltransferase in biosynthesis of ligands for E-selectin and P-selectin.},
  author={Karen R. Snapp and Christine E. Heitzig and Lesley G. Ellies and Jamey D Marth and Geoffrey S Kansas},
  journal={Blood},
  year={2001},
  volume={97 12},
  pages={
          3806-11
        }
}
Selectins are carbohydrate-binding adhesion molecules that play important roles in control of leukocyte traffic. Glycosyltransferases involved in selectin ligand biosynthesis include the alpha1,3-fucosyltransferases FucT-VII and FucT-IV, one or more sialyltransferases, and at least one O-linked branching enzyme. Previous studies have shown that core 2 beta1-6-N-glucosaminyltransferase (C2GlcNAcT-I; EC 2.4.1.102) is required for functional modification of PSGL-1, the leukocyte P-selectin ligand… 
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Functional Analysis of the Combined Role of the O-Linked Branching Enzyme Core 2 β1-6-N-Glucosaminyltransferase and Dimerization of P-selectin Glycoprotein Ligand-1 in Rolling on P-selectin*
TLDR
The contributions of both C2GlcNAcT-I glycosylation and dimerization of PSGL-1 to adhesive bonds formed during tethering and rolling of transfected cell lines on purified P-selectin were measured.
An Alternate Core 2 β1,6-N-Acetylglucosaminyltransferase Selectively Contributes to P-Selectin Ligand Formation in Activated CD8 T Cells1
TLDR
It is demonstrated that multiple C2glcNAcT enzymes can contribute to P-selectin ligand formation and may cooperate with C2Glc NAcT-I in the control of CD8 T cell trafficking.
Competition between Core-2 GlcNAc-transferase and ST6GalNAc-transferase Regulates the Synthesis of the Leukocyte Selectin Ligand on Human P-selectin Glycoprotein Ligand-1*
TLDR
Overexpression of glycoprotein-specific ST6GalNAc-transferases in human promyelocytic HL-60 cells altered glycan structures and cell adhesion properties and suggest that a competition between ST6galNAc2 and C2GnT-1 for the core-1/Galβ1,3GalNAC glycan may regulate leukocyte adhesion under fluid shear.
Core 2 ß1,6-N-acetylglucosaminyltransferase-I, crucial for P-selectin ligand expression is controlled by a distal enhancer regulated by STAT4 and T-bet in CD4+ T helper cells 1.
Formation of functional selectin ligands on activated T cells and thymic progenitors : the role of core 2 β1,6-N-acetylglucosaminyltransferases in the control of lymphocyte trafficking and thymic progenitor homing
TLDR
In vitro and in vivo T cell stimulation conditions that guide P-selectin ligand expression in absence of C2GlcNAcT-I are determined and PSGL-1 expressed on thymic progenitors is shown to be a functionally imperative component of theThymic homing process.
An investigation of the interactions of E-selectin with fuco-oligosaccharides of the blood group family.
This investigation is concerned with assignments of Lewis(a) (Le(a)) and Le(x) analogs on linear and branched di- to hexasaccharide backbones as components of the recognition motifs for E-selectin.…
Cutting Edge: Differential Requirements for Stat4 in Expression of Glycosyltransferases Responsible for Selectin Ligand Formation in Th1 Cells1
TLDR
Data show that FucT-VII and C2GlcNAcT-I are controlled through distinct pathways and imply the existence of at least one other IL-12-inducible glycosyltransferase required for E-selectin and possibly P- selectin ligand formation in Th1 cells.
Affinity and kinetics of sialyl Lewis-X and core-2 based oligosaccharides binding to L- and P-selectin.
Soluble oligosaccharide mimetics of natural selectin ligands act as competitive inhibitors of leukocyte adhesion in models of inflammation. We quantified the binding of simple oligosaccharides based…
with sialyl-Lewis x, which is a functional glycan ligand for P-selectin The monoclonal antibody CHO-131 binds to a core 2 O-glycan terminated
TLDR
CHO-131 staining of neutrophils was diminished following sialomucin removal by O-glycoprotease, and its reactivity with transfected hematopoietic cell lines correlated with the expression of P-selectin ligands.
Specificities ofN-Acetylglucosamine-6-O-sulfotransferases in Relation to L-selectin Ligand Synthesis and Tumor-associated Enzyme Expression*
TLDR
The ability of GlcNAc6ST-1 to sulfate core 2 structure as efficiently as Glc NAc6 ST-2 is consistent with the view that Glcnac6st-1 is also involved in the synthesis of l-selectin ligand.
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TLDR
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