Differential regulation of the Tn10-encoded tetracycline resistance genes tetA and tetR by the tandem tet operators O1 and O2.

@article{Meier1988DifferentialRO,
  title={Differential regulation of the Tn10-encoded tetracycline resistance genes tetA and tetR by the tandem tet operators O1 and O2.},
  author={Ir{\`e}ne Meier and Lewis V. Wray and W. Hillen},
  journal={The EMBO journal},
  year={1988},
  volume={7 2},
  pages={
          567-72
        }
}
The Tn10-encoded tet transcriptional control sequence consists of bidirectional, overlapping promoters which are superimposed by a tandem operator arrangement. Three mutations have been constructed by oligonucleotide-directed mutagenesis which reduce binding of Tet repressor to either one or both of the tandem tet operators 1000-fold as determined by DNAseI footprinting in vitro. The affinity of Tet repressor for mutant tet operators is not affected by the presence of an already occupied… CONTINUE READING
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