We have applied a proteomics approach to analyze signaling cascades in human platelets stimulated by thrombin receptor activating peptide (TRAP). By analyzing basal and TRAP-activated platelets using 2-dimensional gel electrophoresis (2-DE), we detected 62 differentially regulated protein features. From these, 41 could be identified by liquid chromatography… (More)
Figure 1. Experimental design for the differential analysis approach. Proteins were extracted from basal and TRAP-activated platelets and resolved in pI 4-7 and pI 6-11 2-DE gels. After detailed differential image analysis between the different sets of gels, indicated in the figure by double-headed arrows, variant spots were excised and digested. Proteins were identified by LC-MS/MS.