Differential mitotic phosphorylation of proteins of the nuclear pore complex.

@article{Macaulay1995DifferentialMP,
  title={Differential mitotic phosphorylation of proteins of the nuclear pore complex.},
  author={Colin Macaulay and Eva Meier and Douglass J. Forbes},
  journal={The Journal of biological chemistry},
  year={1995},
  volume={270 1},
  pages={254-62}
}
During each cell cycle, the nucleus of higher eukaryotes undergoes a dramatic assembly and disassembly. These events can be faithfully reproduced in vitro using cell-free extracts derived from Xenopus eggs. Such extracts contain three major N-acetylglucosaminylated proteins, p200, p97, and p60. All three become assembled into reconstituted nuclear pores. Here we show that p200, p97, and p60 exist in eggs in soluble high molecular mass complexes of 1000, 450, and 600 kDA, respectively. The bulk… CONTINUE READING
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