Differential metabolism of midazolam in mouse liver and intestine microsomes: a comparison of cytochrome P450 activity and expression.

@article{Perloff2003DifferentialMO,
  title={Differential metabolism of midazolam in mouse liver and intestine microsomes: a comparison of cytochrome P450 activity and expression.},
  author={Michael D. Perloff and Lisa L. von Moltke and David J Greenblatt},
  journal={Xenobiotica; the fate of foreign compounds in biological systems},
  year={2003},
  volume={33 4},
  pages={365-77}
}
1. Although multiple cytochrome P450s (CYP) contribute to hepatic phase I metabolism, CYP3A is the principal subfamily present in human and mouse small intestine. 2. Differences in phase I metabolism were investigated using midazolam (MDZ) hydroxylation in mouse liver and intestinal microsomes. The net MDZ metabolite formation rate in intestinal microsomes was approximately 30% that of liver microsomes (at 250 micro M MDZ). 3. Quantitative Western blotting with anti-CYP3A1 antibody detected two… CONTINUE READING