Differential isotope-labeling for Leu and Val residues in a protein by E. coli cellular expression using stereo-specifically methyl labeled amino acids.

@article{Miyanoiri2013DifferentialIF,
  title={Differential isotope-labeling for Leu and Val residues in a protein by E. coli cellular expression using stereo-specifically methyl labeled amino acids.},
  author={Yohei Miyanoiri and Mitsuhiro Takeda and Kosuke Okuma and Akira Mei Ono and Tsutomu Terauchi and Masatsune Kainosho},
  journal={Journal of biomolecular NMR},
  year={2013},
  volume={57 3},
  pages={237-49}
}
The (1)H-(13)C HMQC signals of the (13)CH3 moieties of Ile, Leu, and Val residues, in an otherwise deuterated background, exhibit narrow line-widths, and thus are useful for investigating the structures and dynamics of larger proteins. This approach, named methyl TROSY, is economical as compared to laborious methods using chemically synthesized site- and stereo-specifically isotope-labeled amino acids, such as stereo-array isotope labeling amino acids, since moderately priced, commercially… CONTINUE READING