Differential involvement of phosphoinositide 3-kinase/Akt in human RPE MCP-1 and IL-8 expression.

@article{Bian2004DifferentialIO,
  title={Differential involvement of phosphoinositide 3-kinase/Akt in human RPE MCP-1 and IL-8 expression.},
  author={Z M Bian and Susan G. Elner and Ayako Yoshida and Victor M. Elner},
  journal={Investigative ophthalmology & visual science},
  year={2004},
  volume={45 6},
  pages={1887-96}
}
PURPOSE To investigate the role of the phosphatidylinositol 3-kinase (PI3K) pathway and the signal mediator AP-1 in monocyte chemotactic protein (MCP)-1 and interleukin (IL)-8 gene expression in human retinal pigment epithelial (hRPE) cells. METHODS hRPE cells were stimulated with IL-1beta and TNF-alpha and by coculturing with monocytes in the presence or absence of a series of kinase inhibitors. The induction of MCP-1 and IL-8 protein and mRNA was determined by ELISA and RT-PCR, respectively… CONTINUE READING