Differential intracellular trafficking, secretion and endosomal localization of two IL-15 isoforms.

@article{Gaggero1999DifferentialIT,
  title={Differential intracellular trafficking, secretion and endosomal localization of two IL-15 isoforms.},
  author={Alessia Gaggero and Bruno Azzarone and Cristina Maria Andrei and Zohar Mishal and Raffaella Meazza and Emanuela Zappia and Anna Rubartelli and Silvano Ferrini},
  journal={European journal of immunology},
  year={1999},
  volume={29 4},
  pages={1265-74}
}
To analyze the intracellular trafficking of two IL-15 isoforms bearing 48- or 21-amino acid leader peptides (L), we have generated cDNA encoding the two proteins fused at the C terminus with green fluorescent protein (GFP). Confocal microscopy analyses showed that, when transfected in CHO cells, 48L IL-15/GFP was localized in the Golgi apparatus and in early endosomes, while 21L IL-15/GFP was detectable only in the cytosol. The presence of 48L IL-15/GFP in endosomes was confirmed by enzyme… CONTINUE READING
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