Differential effects of trilostane and cyanoketone on the 3β-hydroxysteroid dehydrogenase-isomerase reactions in androgen and 16-androstene biosynthetic pathways in the pig testis

@article{Cooke1996DifferentialEO,
  title={Differential effects of trilostane and cyanoketone on the 3$\beta$-hydroxysteroid dehydrogenase-isomerase reactions in androgen and 16-androstene biosynthetic pathways in the pig testis},
  author={Gerard M. Cooke},
  journal={The Journal of Steroid Biochemistry and Molecular Biology},
  year={1996},
  volume={58},
  pages={95-101}
}
  • G. Cooke
  • Published 1996
  • Medicine, Biology
  • The Journal of Steroid Biochemistry and Molecular Biology
3 beta-Hydroxysteroid dehydrogenase-isomerase (3 beta-HSD-I) activity in the pig testis is responsible for the conversion of dehydroepiandrosterone (DHA) to 4-androstenedione and also for the conversion of 5,16-androstadien-3 beta-ol (andien-beta) to 4, 16-androstadien-3-one (dienone). Therefore, 3 beta-HSD-I plays an essential role in the biosynthesis of hormonally and pheromonally active steroids. Previous studies from this laboratory have suggested that the 3 beta-HSD-I reactions in the… Expand
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It is shown that homogenates prepared from rat sciatic nerves contain a functional 3β‐HSD enzyme and its kinetic properties and its regulation by steroids are analysed. Expand
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References

SHOWING 1-10 OF 25 REFERENCES
Kinetic evidence for separate 3β-hydroxysteroid dehydrogenase-isomerases in androgen and 16-androstene biosynthetic pathways in the pig testis
TLDR
It is concluded that substrate specific 3 beta-HSD-isomerases for andien-beta and DHA exist in the immature pig testis and there is little, if any interaction between these enzymes. Expand
Inhibitory effect of synthetic progestins, 4-MA and cyanoketone on human placental 3β-hydroxysteroid dehydrogenase/5→4-ene-isomerase activity
TLDR
Human placental 3 beta-hydroxysteroid dehydrogenase/5----4-ene isomerase (3 beta-HSD) purified from human placenta transforms C-21 as well as C-19 into the corresponding 3-keto- 4-ene-steroids and is thus involved in the biosynthesis of all classes of hormonal steroids. Expand
Studies on the inhibition by 5alpha-pregnane-3,20-dione of the biosynthesis of 16-androstenes and dehydroepiandrosterone in boar testis preparations.
TLDR
Evidence is presented to suggest that the latter steroid may be a physiological regulator of C 19 steroid biosynthesis from pregnenolone and progesterone in boar testis. Expand
Phospholipases modulate immature pig testicular androgen and 16-androstene biosynthetic pathways in vitro
  • G. Cooke
  • Biology, Medicine
  • The Journal of Steroid Biochemistry and Molecular Biology
  • 1992
TLDR
The different effects of phospholipases on these enzymes suggests that pregnenolone metabolism may be regulated by alterations in the membrane microenvironment. Expand
Identification of phospholipids capable of modulating the activities of some enzymes involved in androgen and 16-androstene biosynthesis in the immature pig testis
  • G. Cooke
  • Medicine, Biology
  • The Journal of Steroid Biochemistry and Molecular Biology
  • 1992
TLDR
It is concluded that substrate specific 3 beta-HSD-isomerases exist for androgen and 16-androstene biosynthesis and that phospholipids may play an intrinsic role in their catalytic activity. Expand
17-Hydroxylase and andien-β synthetase activities in immature pig testis microsomal fraction: Kinetic studies of the pregnenolone binding site and possible intermediates of the reactions
  • J. Lavallée, G. Cooke
  • Biology, Medicine
  • The Journal of Steroid Biochemistry and Molecular Biology
  • 1993
The microsomal fraction from the testes of immature pigs can convert pregnenolone to 17-hydroxypregnenolone and also to 5,16-androstadien-3 beta-ol (andien-beta). The available evidence supports theExpand
Inhibition of 16-androstene biosynthesis in boar testis preparations by known and new steroids.
TLDR
The androgen biosynthesis via 17α-hydroxyprogesterone was inhibited to the same extent by these steroids, and the most potent inhibitor was 17β-aminocarbonyloxy-4-androsten-3-one. Expand
Cytochrome b5 promotes the synthesis of delta 16-C19 steroids by homogeneous cytochrome P-450 C21 side-chain cleavage from pig testis.
TLDR
It is concluded that the cytochrome P-450 C21 side-chain cleavage enzyme from pig testicular microsomes is also capable of synthesizing delta 16-C19-steroids and is, therefore, likely to be responsible for the large amounts of the pherormone androstadienone produced by male pigs. Expand
Regulation of expression of male-specific rat liver microsomal 3 beta-hydroxysteroid dehydrogenase.
TLDR
An age-dependent increase in levels of immunoreactive 3 beta HSD is shown through the time of maturation of the male rat, and the apparent Km for liver microsomal NAD(+)-dependent 3beta HSD activity is determined to be 20 microM in both adult male and female liver and was much greater than the Km of rat Leydig tumor 3 betaHSD activity. Expand
Inhibition of human placental progesterone synthesis and aromatase activity by synthetic steroidogenic inhibitors in vitro.
TLDR
The inhibitory effect in vitro of four synthetic steroids on enzyme systems of placental progesterone synthesis at term was analyzed and no difference in the effect of steroids on the 20 alpha-HSDH of early gestational and term placenta was found. Expand
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