Differential effects of the phorbol ester TPA on DNA-mediated transfection in a variety of cell lines.

@article{Reston1993DifferentialEO,
  title={Differential effects of the phorbol ester TPA on DNA-mediated transfection in a variety of cell lines.},
  author={James T. Reston and Susan Gould-Fogerite and Raphael J. Mannino},
  journal={Biochimica et biophysica acta},
  year={1993},
  volume={1177 1},
  pages={49-53}
}
The efficiency of stable gene transfer and expression in NIH3T3 cells has been shown to be significantly enhanced by a brief treatment with the phorbol ester tetradecanoylphorbol 12,13-acetate (TPA) immediately following calcium-phosphate transfection. Several lines of evidence indicated that this effect was mediated through protein kinase C activation… CONTINUE READING