Differential effects of permeating and nonpermeating solutes on the fatty acid composition of Pseudomonas putida.

@article{Halverson2000DifferentialEO,
  title={Differential effects of permeating and nonpermeating solutes on the fatty acid composition of Pseudomonas putida.},
  author={Larry J. Halverson and Mary K. Firestone},
  journal={Applied and environmental microbiology},
  year={2000},
  volume={66 6},
  pages={2414-21}
}
We examined the effect of reduced water availability on the fatty acid composition of Pseudomonas putida strain mt-2 grown in a defined medium in which the water potential was lowered with the permeating solutes NaCl or polyethylene glycol (PEG) with a molecular weight of 200 (PEG 200) or the nonpermeating solute PEG 8000. Transmission electron microscopy showed that -1.0-MPa PEG 8000-treated cells had convoluted outer membranes, whereas -1.0-MPa NaCl-treated or control cells did not. At the… CONTINUE READING

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