Differential effects of ammonia and β-methylene-dl-aspartate on metabolism of glutamate and related amino acids by astrocytes and neurons in primary culture

@article{Lai1989DifferentialEO,
  title={Differential effects of ammonia and β-methylene-dl-aspartate on metabolism of glutamate and related amino acids by astrocytes and neurons in primary culture},
  author={James C. K. Lai and Ch. R. K. Murthy and Arthur J. L. Cooper and Elna Hertz and Leif Hertz},
  journal={Neurochemical Research},
  year={1989},
  volume={14},
  pages={377-389}
}
The effects of ammonium chloride (3 mM) and β-methylene-dl-aspartate (BMA; 5 mM) (an inhibitor of aspartate aminotransferase, a key enzyme of the malate-aspartate shuttle (MAS)) on the metabolism of glutamate and related amino acids were studied in primary cultures of astrocytes and neurons. Both ammonia and BMA inhibited14CO2 production from [U-14C]-and [1-14C]glutamate by astrocytes and neurons and their effects were partially additive. Acute treatment of astrocytes with ammonia (but not BMA… CONTINUE READING
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3MA alters neuronal and astroglial amino acid metabolism

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1 Excerpt

Ammonia and energy metabolism in normal and hyperammonemic rat

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1 Excerpt

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