Differential effects of CTLA-4 substitutions on the binding of human CD80 (B7-1) and CD86 (B7-2).

@article{Morton1996DifferentialEO,
  title={Differential effects of CTLA-4 substitutions on the binding of human CD80 (B7-1) and CD86 (B7-2).},
  author={Phillip A Morton and X T Fu and Jennifer A Stewart and K S Giacoletto and Sheryl L. White and Clinton E. Leysath and Richard J. Evans and J. J. Shieh and Robert W. Karr},
  journal={Journal of immunology},
  year={1996},
  volume={156 3},
  pages={1047-54}
}
CTLA-4 expressed on activated T cells binds to CD80 (B7-1) and CD86 (B7-2) molecules present on APC with high avidity and appears to deliver a negative regulatory signal to the T cell. We have investigated the kinetics of CTLA-4 binding to CD80 and CD86, together with the effects of selected CTLA-4 mutations on binding activity. The dissociation constants (Kd) for binding of CTLA-4-Ig to CD80 and CD86 transfectants were 8.1 and 6.7 nM, respectively. Surface plasmon resonance was used to… CONTINUE READING

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