Differential effects of 6-DMAP, olomoucine and roscovitine on Xenopus oocytes and eggs.

@article{Flament2000DifferentialEO,
  title={Differential effects of 6-DMAP, olomoucine and roscovitine on Xenopus oocytes and eggs.},
  author={St{\'e}phane Flament and J F Bodart and M Bertout and Edith Browaeys and Adrien Rousseau and Jean Pierre Vilain},
  journal={Zygote},
  year={2000},
  volume={8 1},
  pages={3-14}
}
The effects of the new cyclin-dependent kinase inhibitors, roscovitine and olomoucine, on oocytes and eggs of Xenopus laevis were investigated and compared with those of 6-dimethylamino purine (6-DMAP). The inhibitory properties of 6-DMAP, olomoucine and roscovitine towards p34cdc2-cyclin B isolated from Xenopus eggs revealed K-IC50 values of 300, 40 and 10 microM respectively. The three compounds inhibited progesterone-induced maturation with M-IC50 values of 200, 100 and 20 microM. These… CONTINUE READING