Differential effect of the combination methotrexate/(dl)-5-methyltetrahydrofolate on lymphoid malignant and normal bone marrow cells.
@article{Bernabei1992DifferentialEO, title={Differential effect of the combination methotrexate/(dl)-5-methyltetrahydrofolate on lymphoid malignant and normal bone marrow cells.}, author={P. Bernabei and W. Bensinger}, journal={Leukemia}, year={1992}, volume={6 2}, pages={ 131-5 } }
It has been reported that high doses of (dl)-5-methyltetrahydrofolate (mTHF) inhibit the growth of leukemic cell lines. We studied the effect of methotrexate (MTX) and mTHF in combination on the lymphoid cell lines BALM 3, CEM, MOLT 4 and P3HR1 by evaluating the clonogenic cell reduction in a limiting dilution assay. Cells were treated with 0.1 mM MTX for 4 h and 1 mM mTHF for 48 h. The growth of the cell lines was inhibited by 1 mM mTHF, with a clonogenic cell reduction of 3.5 log. MTX… CONTINUE READING
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