Corpus ID: 45790736

Differential effect of the combination methotrexate/(dl)-5-methyltetrahydrofolate on lymphoid malignant and normal bone marrow cells.

@article{Bernabei1992DifferentialEO,
  title={Differential effect of the combination methotrexate/(dl)-5-methyltetrahydrofolate on lymphoid malignant and normal bone marrow cells.},
  author={P A Bernabei and William I. Bensinger},
  journal={Leukemia},
  year={1992},
  volume={6 2},
  pages={
          131-5
        }
}
It has been reported that high doses of (dl)-5-methyltetrahydrofolate (mTHF) inhibit the growth of leukemic cell lines. We studied the effect of methotrexate (MTX) and mTHF in combination on the lymphoid cell lines BALM 3, CEM, MOLT 4 and P3HR1 by evaluating the clonogenic cell reduction in a limiting dilution assay. Cells were treated with 0.1 mM MTX for 4 h and 1 mM mTHF for 48 h. The growth of the cell lines was inhibited by 1 mM mTHF, with a clonogenic cell reduction of 3.5 log. MTX… Expand
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