Differential effect of DNA supercoiling on transcription of adenovirus genes in vitro.

@article{Banerjee1992DifferentialEO,
  title={Differential effect of DNA supercoiling on transcription of adenovirus genes in vitro.},
  author={Sreeparna Banerjee and David J. Spector},
  journal={The Journal of general virology},
  year={1992},
  volume={73 ( Pt 10)},
  pages={2631-8}
}
We examined the effect of DNA template topology on the transcription of immediate early (E1a), early (E1b) and late (pIX) adenovirus genes in vitro. Transcription in whole cell extracts was measured by quantitative hybridization to end-labelled DNA and protection of hybrids from S1 nuclease digestion. Two- to fourfold more E1a RNA was synthesized from supercoiled, compared to linear, DNA templates. Similarly, transcription of the E1b gene was stimulated three- to sevenfold when the template was… CONTINUE READING