Differential dynamics of splicing factor SC35 during the cell cycle

@article{Tripathi2008DifferentialDO,
  title={Differential dynamics of splicing factor SC35 during the cell cycle},
  author={K. Tripathi and V. Parnaik},
  journal={Journal of Biosciences},
  year={2008},
  volume={33},
  pages={345-354}
}
Pre-mRNA splicing factors are enriched in nuclear domains termed interchromatin granule clusters or nuclear speckles. During mitosis, nuclear speckles are disassembled by metaphase and reassembled in telophase in structures termed mitotic interchromatin granules (MIGs). We analysed the dynamics of the splicing factor SC35 in interphase and mitotic cells. In HeLa cells expressing green fluorescent protein (GFP)-SC35, this was localized in speckles during interphase and dispersed in metaphase. In… Expand
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References

SHOWING 1-10 OF 46 REFERENCES
A serine kinase regulates intracellular localization of splicing factors in the cell cycle
TLDR
The results indicate that SRPK1 may have a central role in the regulatory network for splicing, controlling the intranuclear distribution of splicing factors in interphase cells, and the reorganization of nuclear speckles during mitosis. Expand
Differential interaction of splicing snRNPs with coiled bodies and interchromatin granules during mitosis and assembly of daughter cell nuclei
TLDR
It is demonstrated that snRNP associations with coiled bodies and interchromatin granules are differentially regulated during the cell division cycle and suggest that these structures play distinct roles connected with snR NP structure, transport, and/or function. Expand
Disassembly of interchromatin granule clusters alters the coordination of transcription and pre-mRNA splicing
TLDR
The authors' data suggest that the protein–protein interactions responsible for the clustering of interchromatin granules are disrupted when SR proteins are hyperphosphorylated and stabilized whenSR proteins are hypoph phosphorylated, demonstrating that IGC disassembly perturbs coordination between transcription and pre-mRNA splicing in mammalian cell nuclei. Expand
Reduced Mobility of the Alternate Splicing Factor (Asf) through the Nucleoplasm and Steady State Speckle Compartments
TLDR
This study examines the movement of a splicing factor, ASF, green fluorescent fusion protein (ASF–GFP) using time-lapse microscopy and the technique fluorescence recovery after photobleaching and proposes that the nonrandom organization of splicing factors reflects spatial differences in the concentration of relatively immobile binding sites. Expand
Hypophosphorylated SR splicing factors transiently localize around active nucleolar organizing regions in telophase daughter nuclei
TLDR
It is found that upon entry into daughter nuclei, snRNPs and SR proteins do not immediately colocalize in nuclear speckles, and the SR protein kinase Clk/STY colocalizes with SR proteins in NAPs, suggesting that phosphorylation releases SR proteins from N APs and their initial target is transcription sites. Expand
Localization and structure of snRNPs during mitosis. Immunofluorescent and biochemical studies.
TLDR
Immunoprecipitation studies with anti-(U1)RNP and anti-Sm autoantibodies, as well as protein analysis of snRNPs isolated from extracts of mitotic cells, demonstrate that the snRNAs remain associated in a specific manner with the same set of proteins during interphase and mitosis. Expand
Associations between distinct pre‐mRNA splicing components and the cell nucleus.
TLDR
These studies reveal a dynamic pattern of assembly and disassembly of the splicing factor SC‐35 into discrete nuclear structures that colocalize with interchromatin granules and perichromatin fibrils, which may be nuclear organelles involved in the assembly of spliceosomes, or splicing itself. Expand
The dynamics of a pre-mRNA splicing factor in living cells
TLDR
It is concluded that one function of speckles is to supply splicing factors to active genes, demonstrating that the interphase nucleus is far more dynamic in nature than previously assumed. Expand
Rapid, diffusional shuttling of poly(A) RNA between nuclear speckles and the nucleoplasm.
TLDR
Fluorescein-labeled oligo(dT) was introduced into HeLa cells stably expressing a red fluorescent protein chimera of the splicing factor SC35 and allowed to hybridize, and tagged poly(A) RNA was observed to rapidly distribute throughout the entire nucleoplasm and other speckles, regardless of whether the tracking observations were initiated in a speckle or theucleoplasm. Expand
Cyclin-dependent kinases govern formation and maintenance of the nucleolus
TLDR
It is proposed that the mechanisms governing both formation and maintenance of functional nucleoli involve CDK activities and couple the cell cycle to ribosome biogenesis. Expand
...
1
2
3
4
5
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