Differential distributions of two adducin-like protein isoforms in the Drosophila ovary and early embryo

@article{Zaccai1996DifferentialDO,
  title={Differential distributions of two adducin-like protein isoforms in the Drosophila ovary and early embryo},
  author={Mary Zaccai and Howard D. Lipshitz},
  journal={Zygote},
  year={1996},
  volume={4},
  pages={159 - 166}
}
Summary Adducin is a cytoskeletal protein that can function in vitro to bundle F-actin and to control the assembly of the F-actin/spectrin cytoskeletal network. The Drosophila Adducin-like (Add) locus (also referred to as hu-li tai shao (hts)) encodes a family of proteins of which several are homologous to mammalian adducin (Ding et al., Proc. Natl. Acad. Sci. USA90, 2512–16, 1993; Yue & Spradling, Genes Dev.6, 2443–54, 1992). We report the identification of two novel adducin isoforms: a 95… 

Role of Adducin-like (hu-li tai shao) mRNA and protein localization in regulating cytoskeletal structure and function during Drosophila Oogenesis and early embryogenesis.

TLDR
Early embryos produced by swallow or Add-hts mutant females have severe defects in the distribution of F-actin and spectrin as well as abnormalities in nuclear division, nuclear migration, and cellularization.

Phospho-regulated Drosophila adducin is a determinant of synaptic plasticity in a complex with Dlg and PIP2 at the larval neuromuscular junction

TLDR
It is shown that the predominant isoforms of Hts at the NMJ contain the MARCKS-homology domain, which is important for interactions with Discs large (Dlg) and phosphatidylinositol 4,5-bisphosphate (PIP2).

alpha-spectrin is required for germline cell division and differentiation in the Drosophila ovary.

TLDR
It is shown that alpha-spectrin is also required for cyst formation and oocyte differentiation, but that its role in each process is distinct from that of Hts protein.

Drosophila betaHeavy-spectrin is essential for development and contributes to specific cell fates in the eye.

TLDR
The results emphasize the fundamental contribution of the spectrin membrane skeleton to normal development and reveals a critical interplay between the integrity of a cell's membrane skeleton, the structure of cell-cell contacts and cell signaling.

Hts, the Drosophila homologue of adducin, physically interacts with the transmembrane receptor golden goal to guide photoreceptor axons

TLDR
Hts, the Drosophila homologue of Adducin, physically interacts with Gogo's cytoplasmic domain via its head‐neck domain, thereby guiding R axons in growth cone filopodia.

Drosophila sosie functions with βH-Spectrin and actin organizers in cell migration, epithelial morphogenesis and cortical stability

Summary Morphogenesis in multicellular organisms requires the careful coordination of cytoskeletal elements, dynamic regulation of cell adhesion and extensive cell migration. sosie (sie) is a novel

Distinct roles for hu li tai shao and swallow in cytoskeletal organization during Drosophila oogenesis

TLDR
Swa is crucial for the organization of actin networks that lead to the formation of a specialized microtubule population, while Ovhts‐RC acts to modulate spatially restricted actin filament growth at the oocyte cortex, which suggests RNA localization can lead to modifications of both the actin and microtubules cytoskeletons at specific subcellular locales.

RNA localization in development.

TLDR
This review considers RNA localization in the context of animal development with a focus on mRNAs and non-protein-coding RNAs localized in Drosophila, Xenopus, ascidian, zebrafish, and echinoderm oocytes and embryos.

Identification of TER94, an AAA ATPase protein, as a Bam-dependent component of the Drosophila fusome.

TLDR
Evidence is presented that the fly homologue of the transitional endoplasmic reticulum ATPase (TER94) is one such protein that suggests that the fusome cisternae grow by vesicle fusion and are a germ cell modification of endoplasmsic Reticulum.
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A comparison of the spatial distribution of bicoid and Adducin-like transcripts in the maternal-effect RNA-localization mutants exuperantia, swallow, and staufen indicates different genetic requirements for proper localization of these two mRNAs to the anterior pole of the oocyte and early embryo.

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The complete sequence of both subunits of human adducin, alpha (737 amino acids), and beta (726 amino acids) has been deduced by analysis of the cDNAs, suggesting evolution by gene duplication.

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A putative role for adducin is identified, and a calcium- and calmodulin-dependent mechanism whereby higher states of actin association and its interaction with spectrin in the erythrocyte may be controlled is defined.

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TLDR
Drosophila females bearing mutations in a previously undescribed gene, hu-li tai shao [(hts) too little nursing], produced egg chambers that contained fewer than the normal 15 nurse cells and that usually lacked an oocyte, and the hts locus was found to encode a homolog of the mammalian membrane skeletal protein adducin.

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TLDR
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TLDR
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