Differential distributions of two adducin-like protein isoforms in the Drosophila ovary and early embryo

  title={Differential distributions of two adducin-like protein isoforms in the Drosophila ovary and early embryo},
  author={Mary Zaccai and Howard D. Lipshitz},
  pages={159 - 166}
Summary Adducin is a cytoskeletal protein that can function in vitro to bundle F-actin and to control the assembly of the F-actin/spectrin cytoskeletal network. The Drosophila Adducin-like (Add) locus (also referred to as hu-li tai shao (hts)) encodes a family of proteins of which several are homologous to mammalian adducin (Ding et al., Proc. Natl. Acad. Sci. USA90, 2512–16, 1993; Yue & Spradling, Genes Dev.6, 2443–54, 1992). We report the identification of two novel adducin isoforms: a 95… 

Role of Adducin-like (hu-li tai shao) mRNA and protein localization in regulating cytoskeletal structure and function during Drosophila Oogenesis and early embryogenesis.

Early embryos produced by swallow or Add-hts mutant females have severe defects in the distribution of F-actin and spectrin as well as abnormalities in nuclear division, nuclear migration, and cellularization.

Phospho-regulated Drosophila adducin is a determinant of synaptic plasticity in a complex with Dlg and PIP2 at the larval neuromuscular junction

It is shown that the predominant isoforms of Hts at the NMJ contain the MARCKS-homology domain, which is important for interactions with Discs large (Dlg) and phosphatidylinositol 4,5-bisphosphate (PIP2).

alpha-spectrin is required for germline cell division and differentiation in the Drosophila ovary.

It is shown that alpha-spectrin is also required for cyst formation and oocyte differentiation, but that its role in each process is distinct from that of Hts protein.

Drosophila betaHeavy-spectrin is essential for development and contributes to specific cell fates in the eye.

The results emphasize the fundamental contribution of the spectrin membrane skeleton to normal development and reveals a critical interplay between the integrity of a cell's membrane skeleton, the structure of cell-cell contacts and cell signaling.

Hts, the Drosophila homologue of adducin, physically interacts with the transmembrane receptor golden goal to guide photoreceptor axons

Hts, the Drosophila homologue of Adducin, physically interacts with Gogo's cytoplasmic domain via its head‐neck domain, thereby guiding R axons in growth cone filopodia.

Drosophila sosie functions with βH-Spectrin and actin organizers in cell migration, epithelial morphogenesis and cortical stability

Summary Morphogenesis in multicellular organisms requires the careful coordination of cytoskeletal elements, dynamic regulation of cell adhesion and extensive cell migration. sosie (sie) is a novel

Distinct roles for hu li tai shao and swallow in cytoskeletal organization during Drosophila oogenesis

Swa is crucial for the organization of actin networks that lead to the formation of a specialized microtubule population, while Ovhts‐RC acts to modulate spatially restricted actin filament growth at the oocyte cortex, which suggests RNA localization can lead to modifications of both the actin and microtubules cytoskeletons at specific subcellular locales.

RNA localization in development.

This review considers RNA localization in the context of animal development with a focus on mRNAs and non-protein-coding RNAs localized in Drosophila, Xenopus, ascidian, zebrafish, and echinoderm oocytes and embryos.

Identification of TER94, an AAA ATPase protein, as a Bam-dependent component of the Drosophila fusome.

Evidence is presented that the fly homologue of the transitional endoplasmic reticulum ATPase (TER94) is one such protein that suggests that the fusome cisternae grow by vesicle fusion and are a germ cell modification of endoplasmsic Reticulum.



Different genetic requirements for anterior RNA localization revealed by the distribution of Adducin-like transcripts during Drosophila oogenesis.

A comparison of the spatial distribution of bicoid and Adducin-like transcripts in the maternal-effect RNA-localization mutants exuperantia, swallow, and staufen indicates different genetic requirements for proper localization of these two mRNAs to the anterior pole of the oocyte and early embryo.

Primary structure and domain organization of human alpha and beta adducin

The complete sequence of both subunits of human adducin, alpha (737 amino acids), and beta (726 amino acids) has been deduced by analysis of the cDNAs, suggesting evolution by gene duplication.

Drosophilia spectrin. I. Characterization of the purified protein

A protein purified from Drosophila S3 tissue culture cells that has many of the diagnostic features of spectrin from vertebrate organisms appears to be a bona fide member of the spectrin family of proteins.

Erythrocyte adducin: a calmodulin-regulated actin-bundling protein that stimulates spectrin-actin binding

A putative role for adducin is identified, and a calcium- and calmodulin-dependent mechanism whereby higher states of actin association and its interaction with spectrin in the erythrocyte may be controlled is defined.

hu-li tai shao, a gene required for ring canal formation during Drosophila oogenesis, encodes a homolog of adducin.

Drosophila females bearing mutations in a previously undescribed gene, hu-li tai shao [(hts) too little nursing], produced egg chambers that contained fewer than the normal 15 nurse cells and that usually lacked an oocyte, and the hts locus was found to encode a homolog of the mammalian membrane skeletal protein adducin.

Modulation of spectrin–actin assembly by erythrocyte adducin

It is demonstrated that a membrane-skeleton-associated calmodulin-binding protein of erythrocytes, called adducin, binds tightly in vitro to spectrin-actin complexes but with much less affinity either toSpectrin or to actin alone, and is inhibited in its ability to induce the binding of additional spectrin molecules toActin by micromolar concentrations of cal modulin and Ca2+.

Drosophila spectrin. II. Conserved features of the alpha-subunit are revealed by analysis of cDNA clones and fusion proteins

Together these data show that the composition, structure, and binding properties of the spectrin family of proteins have been remarkably well conserved between arthropods and vertebrates.

The Drosophila fusome, a germline-specific organelle, contains membrane skeletal proteins and functions in cyst formation.

The results imply that Drosophila fusomes are required for ovarian cyst formation and suggest that membrane skeletal proteins regulate cystocyte divisions.

Adducin: Ca++-dependent association with sites of cell-cell contact

The discovery that ad Ducin is concentrated at sites of cell-cell contact in the epithelial tissues where it is expressed is consistent with the idea that adducin recognizes and associates with specific "receptors" localized at regions ofcell- cell contact and promotes assembly of spectrin into a more stable structure, perhaps analogous to the highly organized spectrin-actin network of erythrocyte membranes.