Differential distribution of alternatively spliced somatostatin receptor 2 isoforms (sst2A and sst2B) in rat spinal cord

@article{Schulz1998DifferentialDO,
  title={Differential distribution of alternatively spliced somatostatin receptor 2 isoforms (sst2A and sst2B) in rat spinal cord},
  author={Stefan Schulz and Harald Schmidt and Manuela H{\"a}ndel and Matthias Schreff and Volker Höllt},
  journal={Neuroscience Letters},
  year={1998},
  volume={257},
  pages={37-40}
}
We have recently shown that the cytoplasmic tail of the somatostatin receptor sst2 undergoes alternative splicing giving rise to two isoforms, SSt2A and sst2B. In the present study, we have raised polyclonal antibodies that specifically detect either sst2A or sst2B and used these antisera for immunocytochemical localization of the receptor proteins in the rat spinal cord. sst2A-immunoreactivity formed a dense network consisting of neuronal perikarya and dendrites in the superficial layers of… 
Localization of five somatostatin receptors in the rat central nervous system using subtype-specific antibodies
TLDR
There appears to be a high degree of specialization among somatostatin receptor subtypes with regard to their subcellular targeting, and this subtype-selective targeting may be the underlying principal of organization that allows som atostatinergic modulation of neuronal activity via both pre- and postsynaptic mechanisms.
Characterisation of somatostatin sst2 receptor splice variants
TLDR
The localisation of the two receptor isoforms in the central nervous system, the periphery and also in tumour tissue is described and the data on the functional properties of the recombinant splice variants that have been generated so far are summarized and discussed.
Distribution, Targeting, and Internalization of the sst4 Somatostatin Receptor in Rat Brain
TLDR
It is shown that sst4 is strictly somatodendritic and most likely functions in a postsynaptic manner and may have a previously unappreciated function during the neuronal degeneration–regeneration process.
Expression changes of somatostatin receptor subtypes sst2A, sst2B, sst3 and sst4 after a cortical contusion trauma in rats
TLDR
After trauma of an ipsilateral cortical and hippocampal damage in the brain of adult rats was induced by a cortical contusion trauma, subsequent changes of expression of different somatostatin receptor subtypes (sst) were examined.
Somatostatin and its 2A receptor in dorsal root ganglia and dorsal horn of mouse and human: expression, trafficking and possible role in pain
TLDR
The findings highlight a significant and complex role of the SST system in pain signaling and suggest that sst2A may represent a potential pharmacologic target for treatment of neuropathic pain.
Homo- and Heterodimerization of Somatostatin Receptor Subtypes
TLDR
It is shown that the somatostatin receptor (sst) subtypes sst2A and sst3 exist as homodimers at the plasma membrane when expressed in human embryonic kidney 293 cells, and coimmunoprecipitation studies using differentially epitope-tagged receptors are provided, providing direct evidence for heterodimerization of sst 2A andsst3.
Cellular biology of somatostatin receptors
TLDR
Evidence that somatostatin receptors can form homo- and heterodimers and can physically interact with members of the SSTRIP/Shank/ProSAP1/CortBP1 family is discussed.
Involvement of Spinal Somatostatin Receptor SST2A in Inflammation-Induced Thermal Hyperalgesia: Ultrastructural and Behavioral Studies in Rats
TLDR
The results suggest that spinal SST2A receptors play a key role in triggering the generation, but not maintenance, of thermal hyperalgesia evoked by CFA-induced inflammation.
Role of Somatostatin and Somatostatin Receptor type 2 in Postincisional Nociception in Rats
TLDR
SST appears to contribute to postincisional pain, and this finding could be of clinical relevance.
Somatostatin 2 Receptors in the Spinal Cord Tonically Restrain Thermogenic, Cardiac and Other Sympathetic Outflows
TLDR
The distribution of SSTR2 within the thoracic spinal cord is investigated and it is revealed that SST tonically mantains sympathetic, cardiovascular and thermogenic functions, as activity in all measured parameters increased.
...
1
2
3
4
...

References

SHOWING 1-10 OF 18 REFERENCES
Immunocytochemical localization of somatostatin receptor sst2A in the rat spinal cord and dorsal root ganglia
TLDR
The present results not only provide a morphological substrate for spinal octreotide analgesia but also show that somatostatin and opioids are poised to modulate nociceptive transmission by distinct anatomical systems.
Cloning and expression of a novel mouse somatostatin receptor (SSTR2B)
TLDR
A mouse somatostatin receptor cDNA was cloned from neuroblastoma x glioma (NG108‐15) cells and encodes a protein which is 23 residues shorter than that predicted from the SSTR2 sequence, and differs in 15 amino acids at the C‐terminus.
Immunolocalization of two mu-opioid receptor isoforms (MOR1 and MOR1B) in the rat central nervous system
TLDR
Evidence is provided that the mu receptor isoforms, MOR1 and MOR1B, exhibit a strikingly different distribution in that MOR1 appears to be the major isoform widely distributed throughout the central nervous system and MOR 1B being predominantly localized to the olfactory bulb.
Somatostatin 2A receptor is expressed by enteric neurons, and by interstitial cells of Cajal and enterochromaffin‐like cells of the gastrointestinal tract
TLDR
The multiple types of cells expressing the sst2A receptor, including enteric neurons and non‐neuronal structures, in addition to the relationship between somatostatin and sst 2A receptor elements, provide evidence that the sSt2A receptors mediates somatstatin effects in the gastrointestinal tract via neuronal and paracrine pathways.
Somatostatin receptors: Distribution in rat central nervous system and human frontal cortex
TLDR
Using radioiodinated analogs of SS‐14 and SS‐28, specific binding sites in rat and human brain sections that display pharmacological properties anticipated for somatostatin receptors and discrete patterns of anatomical localization are identified.
Alternative splicing generates metabotropic glutamate receptors inducing different patterns of calcium release in Xenopus oocytes.
TLDR
In situ hybridization data show that mGLUR1c mRNA is expressed at a lower level than the other splice variants of mGluR1, and some differences in the regional distribution of these transcripts were observed in the cerebellum, the olfactory bulb, and the striatum.
Classification and nomenclature of somatostatin receptors.
TLDR
It is acknowledged that much more data from endogenous receptors in whole tissues are needed before further recommendations on somatostatin receptor nomenclature can be made, but a promising approach is discussed.
Characterization of two alternatively spliced forms of a metabotropic glutamate receptor in the central nervous system of the rat
TLDR
The presence of mGluR1 alpha in cells of the hippocampus that are associated with the release of somatostatin, suggest that this receptor could play a role in regulating hippocampal excitability in both normal and epileptic tissues.
A quantitative autoradiographical study on the distribution of somatostatin sst2 receptors in the rat central nervous system using [125I]-BIM-23027
TLDR
The widespread distribution of [125I]-BIM-23027 binding sites within the brain suggests that receptors similar to the recombinant sst2 receptor may mediate a variety of different physiological effects within the central nervous system.
Differential cellular localization of three splice variants of the mGluR1 metabotropic glutamate receptor in rat cerebellum.
TLDR
Antibodies were raised against C-terminal peptides of the splice variants a, b and c of the rat mGluR1 metabotropic glutamate receptor to study the cellular localization of these receptors in rat cerebellum.
...
1
2
...