Differential dependence on cysteine from transsulfuration versus transport during T cell activation.

Abstract

The synthesis of glutathione, a major cellular antioxidant with a critical role in T cell proliferation, is limited by cysteine. In this study, we evaluated the contributions of the x(C)(-) cystine transporter and the transsulfuration pathway to cysteine provision for glutathione synthesis and antioxidant defense in naïve versus activated T cells and in the immortalized T lymphocyte cell line, Jurkat. We show that the x(C)(-) transporter, although absent in naïve T cells, is induced after activation, releasing T cells from their cysteine dependence on antigen-presenting cells. We also demonstrate the existence of an intact transsulfuration pathway in naïve and activated T cells and in Jurkat cells. The flux through the transsulfuration pathway increases in primary but not in transformed T cells in response to oxidative challenge by peroxide. Inhibition of the transsulfuration pathway in both primary and transformed T cells decreases cell viability under oxidative-stress conditions.

DOI: 10.1089/ars.2010.3496

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@article{Garg2011DifferentialDO, title={Differential dependence on cysteine from transsulfuration versus transport during T cell activation.}, author={Sanjay Kumar Garg and Zhonghua Yan and Victor M. Vitvitsky and Ruma Banerjee}, journal={Antioxidants & redox signaling}, year={2011}, volume={15 1}, pages={39-47} }