Differential cleavage and developmental rates and their correlation with cell numbers and sex ratios in buffalo embryos generated in vitro.

@article{Totey1996DifferentialCA,
  title={Differential cleavage and developmental rates and their correlation with cell numbers and sex ratios in buffalo embryos generated in vitro.},
  author={Satish M Totey and Morteza Sam Daliri and K B Appa Rao and C H Pawshe and Maneesh Taneja and Ram Chillar},
  journal={Theriogenology},
  year={1996},
  volume={45 2},
  pages={521-33}
}
In vitro matured and fertilized buffalo oocytes were co-cultured with buffalo oviductal epithelial cells (BOEC) in CRlaa medium. Cleaved embryos were separated according to the time of completion of first cleavage (i.e., before 30 h and after 30 h post insemination) and cultured for 5 to 10 d and allowed to develop to the blastocyst stage. Zygotes cleaving before 30 h were termed fast-cleaving while those cleaving after 30 h were termed slow-cleaving. The results indicated that fast-cleaving… CONTINUE READING