Differential association of microRNAs with polysomes reflects distinct strengths of interactions with their mRNA targets.

@article{Molotski2012DifferentialAO,
  title={Differential association of microRNAs with polysomes reflects distinct strengths of interactions with their mRNA targets.},
  author={Natali Molotski and Yoav Soen},
  journal={RNA},
  year={2012},
  volume={18 9},
  pages={
          1612-23
        }
}
While microRNAs have been shown to copurify with polysomes, their relative fraction in the translation pool (polysome occupancy) has not yet been measured. Here, we introduce a high-throughput method for quantifying polysome occupancies of hundreds of microRNAs and use it to investigate factors affecting these occupancies. Analysis in human embryonic stem cells (hESCs) and foreskin fibroblasts (hFFs) revealed microRNA-specific preferences for low, medium, or high polysome occupancy… 

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