Corpus ID: 36947301

Differential antibody responses to Corynebacterium pseudotuberculosis in sheep with naturally acquired caseous lymphadenitis.

  title={Differential antibody responses to Corynebacterium pseudotuberculosis in sheep with naturally acquired caseous lymphadenitis.},
  author={John A. Ellis and D A Hawk and Larry D. Holler and Kenneth W. Mills and Diana L. Pratt},
  journal={Journal of the American Veterinary Medical Association},
  volume={196 10},
Enzyme-linked immunosorbent assays (ELISA) with Corynebacterium pseudotuberculosis cell wall and bacteria-free supernatant with exotoxin preparations as antigens, and hemolysis inhibition tests were used to detect antibodies in the sera of adult range sheep with naturally acquired caseous lymphadenitis (CL). The extent and severity of lesions were quantitated on the basis of a lesion score, derived from an examination of the carcass (peripheral lymphoid tissue) and viscera (including internal… Expand
Serological studies on Corynebacterium pseudotuberculosis infections in goats using enzyme-linked immunosorbent assay.
  • R. Sting, G. Steng, D. Spengler
  • Biology, Medicine
  • Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B
  • 1998
Corynebacterium pseudotuberculosis was isolated from a goat suffering from caseous lymphadenitis and used for preparation of cell wall antigens and exotoxin for detection of specific antibodies inExpand
Experimental Corynebacterium pseudotuberculosis primary infection in goats: kinetics of IgG and interferon-gamma production, IgG avidity and antigen recognition by Western blotting.
Both humoral and cellular responses kinetics presented a similar pattern of activation/deactivation but necropsy results suggested that the IFN-gamma test would be a very specific marker of CLA status. Expand
Corynebacterium pseudotuberculosis primary infection in goats : kinetics of IgG and interferon-g production , IgG avidity and antigen recognition by Western blotting
Corynebacterium pseudotuberculosis is the cause of caseous lymphadenitis (CLA) in small ruminants, a chronic granulomatous disease that provokes significant zootechnics losses to ovine and goatExpand
Antigen specificity of antibody responses to Corynebacterium pseudotuberculosis in naturally infected sheep with caseous lymphadenitis.
There was no apparent relationship between the stage of disease and the response to specific corynebacterial antigens in these animals. Expand
Performance of a whole blood interferon-gamma assay for detection and eradication of caseous lymphadenitis in sheep.
The whole blood IFN-gamma assay shows promise for eradication of caseous lymphadenitis from sheep flocks, with a sensitivity of 91% and a specificity of 98% and postmortem data supported the results in test-negative animals. Expand
Diagnostic comparison of Corynebacterium pseudotuberculosis through microbiological culture and PCR in sheep samples
It is concluded that molecular diagnosis (PCR) in clinical samples proved to be more efficient, reproducible, and faster than the microbiological culture, both on clinical samples analyses and in the confirmation of C. pseudotuberculosis diagnosis. Expand
Control of caseous lymphadenitis in six sheep flocks using clinical examination and regular ELISA testing
In an effort to control the spread of caseous lymphadenitis (CLA) infection, flocks of affected sheep on six holdings were tested serologically at regular intervals using an antibody ELISA with aExpand
Serology and clinical relevance of Corynebacterium pseudotuberculosis in native Korean goats (Capra hircus coreanae)
Seroprevalence and bacterial isolation rate from most of the superficial CLA lesions in native Korean goats are high, suggesting that many internal CLA lesions exist in this goat population, and appropriate control programs need to be established. Expand
Clinical, humoral, and pathologic findings in adult alpacas with experimentally induced Corynebacterium pseudotuberculosis infection.
Alpacas inoculated with C pseudotuberculosis developed abscesses at the inoculation site and internally in the renal lymph nodes, without lung lesions, and Corynebacterium pseudotubculosis isolates from llama and alpaca origin were found to be pathogenically indistinct. Expand
Haptoglobin and fibrinogen concentrations and leukocyte counts in the clinical investigation of caseous lymphadenitis in sheep.
Serum haptoglobin concentration and monocyte counts may be potential markers for progression of CLA in sheep. Expand