Differential activities of H+extrusion systems in MDCK cells due to extracellular osmolality and pH.

@article{Feifel1997DifferentialAO,
  title={Differential activities of H+extrusion systems in MDCK cells due to extracellular osmolality and pH.},
  author={Elisabeth Feifel and M. E. Krall and John P. Geibel and Walter Pfaller},
  journal={The American journal of physiology},
  year={1997},
  volume={273 4 Pt 2},
  pages={F499-506}
}
The aim of the present study was to obtain detailed information on MDCK cell proton secretion characteristics under various growth conditions. Confluent monolayers cultured on glass coverslips were adapted over 48 h to media with different osmolality and pH (200 mosmol/kgH2O, pH 7.4; 300 mosmol/kgH2O, pH 7.4; and 600 mosmol/kgH2O, pH 6.8) corresponding to the luminal fluid composition of the collecting duct segments found in the in renal cortex, the outer stripe of outer medulla and inner… CONTINUE READING