Differential Stoichiometry among Core Ribosomal Proteins

@article{Slavov2015DifferentialSA,
  title={Differential Stoichiometry among Core Ribosomal Proteins},
  author={Nikolai Slavov and Stefan Semrau and Edoardo M. Airoldi and Bogdan Budnik and Alexander van Oudenaarden},
  journal={Cell Reports},
  year={2015},
  volume={13},
  pages={865 - 873}
}

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References

SHOWING 1-10 OF 137 REFERENCES
Functional specialization of ribosomes?
  • W. Gilbert
  • Biology
    Trends in biochemical sciences
  • 2011
How common are extraribosomal functions of ribosomal proteins?
Quantitative proteomic analysis of ribosome assembly and turnover in vivo.
Ribosome-omics of the human ribosome.
TLDR
It is shown that for 80%-90% of the RP genes, the molar ratio of mRNAs varies less than threefold, with little tissue specificity, and since the RPs are needed in equimolar amounts, there must be sluggish or regulated translation of the more abundant RP m RNAs and/or substantial turnover of unused RPs.
Characterization of the ribosome biogenesis landscape in E. coli using quantitative mass spectrometry.
Specialized ribosomes: a new frontier in gene regulation and organismal biology
TLDR
The hypothesis that intrinsic regulation by the ribosome acts to selectively translate subsets of mRNAs harbouring unique cis-regulatory elements, thereby introducing an additional level of regulation in gene expression and the life of an organism is discussed.
Ribosome biogenesis: of knobs and RNA processing.
The Ribosomal Protein Rpl22 Controls Ribosome Composition by Directly Repressing Expression of Its Own Paralog, Rpl22l1
TLDR
It is proposed that ribosome specificity may exist in mammals, providing evidence that one ribosomal protein can influence composition of the ribosomes by regulating its own paralog.
...
...