Differential Staining of Bacteria: Acid Fast Stain

@article{Reynolds2009DifferentialSO,
  title={Differential Staining of Bacteria: Acid Fast Stain},
  author={Jackie Reynolds and Rita B. Moyes and Donald P. Breakwell},
  journal={Current Protocols in Microbiology},
  year={2009},
  volume={15}
}
Acid‐fastness is an uncommon characteristic shared by the genera Mycobacterium (Section 10A) and Nocardia. Because of this feature, this stain is extremely helpful in identification of these bacteria. Although Gram positive, acid‐fast bacteria do not take the crystal violet into the wall well, appearing very light purple rather than the deep purple of normal Gram‐positive bacteria. Curr. Protoc. Microbiol. 15:A.3H.1‐A.3H.5. © 2009 by John Wiley & Sons, Inc. 
View on Wiley
ncbi.nlm.nih.gov
14 Citations
Background Citations
3
Methods Citations
1

14 Citations

Citation Type

Citation Type

Acid Fast Bacteria
TLDR
The use of highly advanced molecular diagnostic tests have come into play, the value of this staining technique cannot be overstated, especially for low and middle-income countries.
Mycobacterium helveticum sp. nov., a novel slowly growing mycobacterial species associated with granulomatous lesions in adult swine
TLDR
A novel slow-growing mycobacterial species isolated from lymph nodes obtained from two sows housed in different Swiss farms is described.
Bedside Diagnostics for Infections: A Guide for Dermatologists
TLDR
Bedside diagnostic tests, such as the Tzanck smear, potassium hydroxide (KOH) preparation, and mineral oil preparation, are discussed, with a specific focus on their use in diagnosing infectious dermatoses.
Mycobacterium avium: an Emerging Pathogen for Dog Breeds with Hereditary Immunodeficiencies
TLDR
The collaborative efforts by microbiologists, veterinary clinicians, dog breeders, primary care physicians, and infectious disease specialists applying the One Health approach are crucial for the best management and prevention of MAC infection.
Development of multiplex PCR and multi-color fluorescent in situ hybridization (m-FISH) coupled protocol for detection and imaging of multi-pathogens involved in inflammatory bowel disease
TLDR
A 4-h multiplex PCR protocol, which was validated by m-FISH images, capable of detecting up to four genes from major pathogens associated with CD, should serve as an excellent tool to support efforts to study multi-pathogens involved in CD and other autoimmune disease.
Evaluation of crystal violet decolorization assay and resazurin microplate assay for antimycobacterial screening
Probiotic characterization and cholesterol assimilation ability of Pichia kudriavzevii isolated from the gut of the edible freshwater snail “Pila globosa”.
TLDR
Probiotics, with cholesterol lowering ability, when supplemented through food can be helpful in reducing the risk of cardiovascular disease and diabetes.
Monoclonal antibodies from humans with Mycobacterium tuberculosis exposure or latent infection recognize distinct arabinomannan epitopes
TLDR
This work generated human IgG mAbs to AM/LAM from B cells of two asymptomatic individuals exposed to or latently infected with M. tuberculosis to enhance the understanding of the spectrum of antibodies to AM-LAM epitopes in humans and are valuable for tuberculosis diagnostic and research applications.
Distinguishing nontuberculous mycobacteria from Mycobacterium tuberculosis lung disease from CT images using a deep learning framework
TLDR
The efficacy of 3D-ResNet as a rapid auxiliary diagnostic tool for NTB-LD and MTB-LD is shown, which can help provide timely and accurate treatment strategies to patients with these diseases.
Characterisation of immune responses to mycobacterial infections in a murine model of type 2 diabetes
TLDR
It is suggested that feeding an energy-dense diet for 30 weeks can induce a T2D phenotype in mice, as well as metabolic and biochemical parameters including body weight, fasting blood glucose level, glucose tolerance test (GTT), glycated haemoglobin level, urine albumin, creatinine and albumin Creatinine ratio (ACR) were assessed after 25 and 30 weeks.
...
1
2
...

References

SHOWING 1-3 OF 3 REFERENCES
Milestones in microbiology 1546 to 1940
Spontaneous Generation and Fermentation The Germ Theory of Disease Immunology Virology Chemotherapy General Microbiology
manual/TOC Online lab manual of this section's author
  • Clinical Infectious Diseases: A Practical Approach
  • 1999
MicrobeLibrary: Acid-fast protocol