Differential Requirements for MCM Proteins in DNA Replication in Drosophila S2 Cells

@article{Crevel2007DifferentialRF,
  title={Differential Requirements for MCM Proteins in DNA Replication in Drosophila S2 Cells},
  author={Gilles Crevel and Reina Hashimoto and Sharron Vass and Jake Sherkow and Masamitsu Yamaguchi and M. Heck and Sue Cotterill},
  journal={PLoS ONE},
  year={2007},
  volume={2},
  pages={1067 - 1075}
}
BACKGROUND The MCM2-7 proteins are crucial components of the pre replication complex (preRC) in eukaryotes. Since they are significantly more abundant than other preRC components, we were interested in determining whether the entire cellular content was necessary for DNA replication in vivo. METHODOLOGY/PRINCIPLE FINDINGS We performed a systematic depletion of the MCM proteins in Drosophila S2 cells using dsRNA-interference. Reducing MCM2-6 levels by >95-99% had no significant effect on cell… CONTINUE READING
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