Differential Protein Expression Analysis of Leishmania major Reveals Novel Roles for Methionine Adenosyltransferase and S-Adenosylmethionine in Methotrexate Resistance*

@article{Drummelsmith2004DifferentialPE,
  title={Differential Protein Expression Analysis of Leishmania major Reveals Novel Roles for Methionine Adenosyltransferase and S-Adenosylmethionine in Methotrexate Resistance*},
  author={Jolyne Drummelsmith and Isabelle L. Girard and Nathalie Trudel and Marc Ouellette},
  journal={Journal of Biological Chemistry},
  year={2004},
  volume={279},
  pages={33273 - 33280}
}
Leishmania is a trypanosomatid parasite causing serious disease and displaying resistance to various drugs. Here, we present comparative proteomic analyses of Leishmania major parasites that have been either shocked with or selected in vitro for high level resistance to the model antifolate drug methotrexate. Numerous differentially expressed proteins were identified by these experiments. Some were associated with the stress response, whereas others were found to be overexpressed due to genetic… Expand
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