Differential Modulation of Gene Expression Encoding Hepatic and Renal Xenobiotic Metabolizing Enzymes by an Aspalathin-Enriched Rooibos Extract and Aspalathin.

  title={Differential Modulation of Gene Expression Encoding Hepatic and Renal Xenobiotic Metabolizing Enzymes by an Aspalathin-Enriched Rooibos Extract and Aspalathin.},
  author={S. Abrahams and S. Samodien and M. Lilly and E. Joubert and W. Gelderblom},
  journal={Planta medica},
  volume={85 1},
Modulation of the expression of hepatic and renal genes encoding xenobiotic metabolizing enzymes by an aspalathin-enriched green rooibos (Aspalathus linearis) extract (GRE) was investigated in the liver and kidneys of F344 rats following dietary exposure of 28 d, as well as selected xenobiotic metabolizing genes in rat primary hepatocytes. In the liver, GRE upregulated genes (p < 0.05) encoding aldehyde dehydrogenase, glucose phosphate isomerase, and cytochrome P450 while 17β-hydroxysteroid… Expand
Differential Cytotoxicity of Rooibos and Green Tea Extracts against Primary Rat Hepatocytes and Human Liver and Colon Cancer Cells - Causal Role of Major Flavonoids.
Differential cell responses were noticed with HepG2 cells more sensitive than PH toward the induction of apoptosis by GRE, and HT-29 cells were resistant to apoptosis induction by the tea and pure flavonoids. Expand
Aspalathin: a rare dietary dihydrochalcone from Aspalathus linearis (rooibos tea)
Aspalathin (2′,3,4,4′,6′-pentahydroxy-3′- C - β -D-glucopyranosyldihydrochalcone) is a natural C -linked glucosyl dihydrochalcone present in Aspalathus linearis (Burm.f.) R.Dahlgren (rooibos), aExpand


Modulation of hepatic drug metabolizing enzymes and oxidative status by rooibos (Aspalathus linearis) and Honeybush (Cyclopia intermedia), green and black (Camellia sinensis) teas in rats.
Modulation of phase II drug metabolizing enzymes and oxidative status in the liver may be important events in the protection against adverse effects related to mutagenesis and oxidative damage. Expand
Xenobiotic Metabolizing Enzymes of the Kidney
Many of the enzymes discussed, in addition to metabolizing foreign compounds, have important endogenous functions in the kidney, such as the regulation of salt and water balance and the synthesis of vitamin D. Expand
Induction of phase I, II and III drug metabolism/transport by xenobiotics
Drug metabolizing enzymes (DMEs) play central roles in the metabolism, elimination and detoxification of xenobiotics and drugs introduced into the human body, and play crucial roles in response to many polycyclic aromatic hydrocarbon receptors and excretion. Expand
Inhibitory Interactions of Aspalathus linearis (Rooibos) Extracts and Compounds, Aspalathin and Z-2-(β-d-Glucopyranosyloxy)-3-phenylpropenoic Acid, on Cytochromes Metabolizing Hypoglycemic and Hypolipidemic Drugs
The findings suggest that herb-drug interactions may occur when nutraceuticals containing rooibos extracts are co-administered with hypoglycemic drugs such as TZDs, sulfonylureas, and dyslipidemic drug, atorvastatin. Expand
Rooibos Flavonoids Inhibit the Activity of Key Adrenal Steroidogenic Enzymes, Modulating Steroid Hormone Levels in H295R Cells
Structural differences regarding the number and position of hydroxyl and glucose moieties as well as structural flexibility could indicate different mechanisms by which these flavonoids influence the activity of adrenal steroidogenic enzymes. Expand
Potential of rooibos, its major C-glucosyl flavonoids, and Z-2-(β-D-glucopyranosyloxy)-3-phenylpropenoic acid in prevention of metabolic syndrome
The potential of rooibos extract and its major C-glucosyl flavonoids, in particular aspalathin, a C- glucoside dihydrochalcone, as well as the phenolic precursor, Z-2-(β-D-Glucopyranosyloxy)-3-phenylpropenoic acid, to prevent the metabolic syndrome, will be highlighted. Expand
Structures of Human Cytochrome P-450 2E1
These structures provide insights into the ability of CYP2E1 to effectively bind and metabolize both small molecule substrates and fatty acids. Expand
The human cytochrome P450 sub-family: transcriptional regulation, inter-individual variation and interaction networks.
  • N. Plant
  • Biology, Medicine
  • Biochimica et biophysica acta
  • 2007
The role of nuclear receptors in regulating CYP3A gene expression, and indeed that of many other proteins involved in drug metabolism, will be examined and will show the need to utilize a systems biology approach to understand fully how the human body responds to chemical exposure. Expand
Interactions of xenobiotics with the steroid hormone biosynthesis pathway
Environmental contaminants can potentially disrupt endocrine processes by interfering with the function of enzymes involved in steroid synthesis and metabolism. Such interferences may result inExpand
Catechol-O-methyltransferase (COMT)-mediated metabolism of catechol estrogens: comparison of wild-type and variant COMT isoforms.
The oxidative metabolism of 17beta-estradiol (E2) and estrone (E1) to catechol estrogens (2-OHE2, 4-OHE2, 2-OHE1, and 4-OHE1) and estrogen quinones has been postulated to be a factor in mammaryExpand