Differential Metabolism of Exogenous and Endogenous Arachidonic Acid in Human Neutrophils*

@article{Sala1999DifferentialMO,
  title={Differential Metabolism of Exogenous and Endogenous Arachidonic Acid in Human Neutrophils*},
  author={Angelo Sala and Simona Zarini and Giancarlo Folco and Robert C. Murphy and Peter M. Henson},
  journal={The Journal of Biological Chemistry},
  year={1999},
  volume={274},
  pages={28264 - 28269}
}
Leukotrienes can be produced by cooperative interactions between cells in which, for example, arachidonate derived from one cell is oxidized to leukotriene A4(LTA4) by another and this can then be exported for conversion to LTB4 or cysteinyl leukotrienes (cys-LTs) by yet another. Neutrophils do not contain LTC4 synthase but are known to cooperate with endothelial cells or platelets (which do have this enzyme) to generate cys-LTs. Stimulation of human neutrophils perfusing isolated rabbit hearts… Expand
Transcellular biosynthesis of cysteinyl leukotrienes in vivo during mouse peritoneal inflammation
TLDR
Transcellular cysLT synthesis during zymosan-induced peritonitis is examined, using bone marrow transplants with transgenic mice deficient in key enzymes of LT synthesis and analyzing all eicosanoids by liquid chromatography/tandem mass spectrometry to highlight the potential relevance of transcellular exchange of LTA4 for the synthesis of LTs mediating biological activities during inflammatory events in vivo. Expand
Cysteinyl leukotriene overproduction in aspirin-exacerbated respiratory disease is driven by platelet-adherent leukocytes.
TLDR
A disturbance in platelet-leukocyte interactions may be partly responsible for the respiratory tissue inflammation and the overproduction of cysLTs that characterize AERD. Expand
The cellular biology of eosinophil eicosanoid formation and function.
TLDR
The local generation of eicosanoids at distinct sites within eosinophils may be important for the roles of these eicOSanoids, both as paracrine mediators pertinent to inflammation and as intracrine signal-transducing mediators that help regulate cellular responses of eos inophils. Expand
Group V Phospholipase A2 Induces Leukotriene Biosynthesis in Human Neutrophils through the Activation of Group IVA Phospholipase A2 *
TLDR
The results indicate that a main role of exogenously added human group V phospholipase A2(hVPLA2) in neutrophil activation and LTB4 biosynthesis is to activate cPLA2 and 5-lipoxygenase primarily by liberating from the outer plasma membrane lyso-PC that induces [Ca2+] i increase and c PLA2 phosphorylation. Expand
Arachidonic acid in postshock mesenteric lymph induces pulmonary synthesis of leukotriene B4.
TLDR
Data indicate that splanchnic ischemia/reperfusion activates gut PLA(2)-mediated release of AA into the lymph where it is delivered to the lungs, provoking LTB(4) production and subsequent PMN-mediated lung injury. Expand
Neutrophil-Endothelial Cell Interaction: A Multidirectional Cross-Talk in Leukotriene Generation
The interaction between neutrophils (PMNLs)-monocytes and endothelial cells (ECs) represents the initial event in the acute inflammatory response and in the pathogenesis of vascular diseases [1]. TheExpand
Transcellular biosynthesis of eicosanoid lipid mediators.
TLDR
In vitro studies, complex functional models, and in vivo evidences of the transcellular biosynthesis of eicosanoids are reviewed and the biological relevance of the metabolites resulting from this unique biosynthetic pathway is reviewed. Expand
Eosinophils and cysteinyl leukotrienes.
TLDR
The mechanisms governing eosinophil LTC(4) synthesis are reviewed, focusing on the distinct intracellular domains that regulate eicosanoid formation and function within eos inophils. Expand
Arachidonic acid cascade in endothelial pathobiology.
TLDR
Endothelial-specific synthesis and action of arachidonic acid derivatives with a particular focus on the mechanisms of signal transduction and associated intracellular protein targets are discussed. Expand
Phosphorylation of serine 523 on 5-lipoxygenase in human B lymphocytes.
TLDR
It is demonstrated by immunoblotting that native 5-LO in mantle B cell lymphoma (MCL) cells (Granta519, JEKO1, and Rec1) and in primary chronic B lymphocytic leukemia cells (B-CLL) is phosphorylated on Ser523 and for the first time a chemical difference between 5- LO in myeloid cells and B cells is demonstrated. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 23 REFERENCES
Transcellular sulfidopeptide leukotriene biosynthetic capacity of vascular cells.
TLDR
It is suggested that both endothelial cells and platelets, in the presence of neutrophils, contribute large quantities of sulfidopeptide leukotrienes to inflammatory and thrombotic situations. Expand
Transcellular metabolism of neutrophil-derived leukotriene A4 by human platelets. A potential cellular source of leukotriene C4.
TLDR
The data indicate that platelets can produce large amounts of LTC4 from neutrophil-derived LTA4 and suggest that such interactions may occur in vivo and that platelet contribution could be an important contribution to the generation of the biologically active L TC4. Expand
Endothelial cell leukotriene C4 synthesis results from intercellular transfer of leukotriene A4 synthesized by polymorphonuclear leukocytes.
TLDR
The data indicate that endothelial cells synthesize LTC4 from LTA4, and demonstrate a specific PMNL-endothelial cell interaction in which endothelial cell L TC4 synthesis results from the intercellular transfer of L TA4 produced by PMNL. Expand
Time-dependent utilization of platelet arachidonic acid by the neutrophil in formation of 5-lipoxygenase products in platelet-neutrophil co-incubations.
TLDR
Results from these experiments suggest different pools of 5-lipoxygenase in the neutrophil and indicate a time and source dependent modulation of arachidonic acid metabolism in mixed cell interactions. Expand
Release of Leukotriene A4 Versus Leukotriene B4 from Human Polymorphonuclear Leukocytes*
TLDR
The objective of this study was to determine the relative amount of leukotriene A4 synthesized by human polymorphonuclear leukocytes (PMNL) that is available for transcellular biosynthetic processes, and it was demonstrated that in human PMNL, the relative amounts of nonenzymatic versus enzymatic leuko A4-derived metabolites increased with decreasing cell concentrations. Expand
Release of platelet-activating factor and the metabolism of leukotriene B4 by the human neutrophil when studied in a cell superfusion model.
TLDR
Comparisons of A23187-induced production of PAF and LTB4 by human neutrophils in a concentrated cell suspension, a diluted cell suspension and in a system in which the cells are placed on a matrix and superfused with buffer at a constant flow rate suggest that a large proportion ofPAF synthesized by neutrophil may be released. Expand
Requirement of a 5-lipoxygenase-activating protein for leukotriene synthesis
TLDR
It is demonstrated that the expression of both the MK-886-binding protein and 5-lipoxygenase is necessary for leukotriene synthesis in intact cells. Expand
Nitric oxide modulation of transcellular biosynthesis of cys‐leukotrienes in rabbit leukocyte‐perfused heart
TLDR
It is suggested that NO provides cardioprotection in an organ model of transcellular metabolism of cys‐LT by preventing PMNL adhesion to the coronary intima by preventing l‐arginine. Expand
Formation of sulphidopeptide‐leukotrienes by cell‐cell interaction causes coronary vasoconstriction in isolated, cell‐perfused heart of rabbit
TLDR
This study suggests that challenge of PMNL present within the coronary vasculature, causes a LTD4‐dependent coronary vasoconstriction, favoured by an efficient uptake of PM NL‐derived LTA4 by endothelial cells. Expand
Morphological and functional changes of coronary vasculature caused by transcellular biosynthesis of sulfidopeptide leukotrienes in isolated heart of rabbit.
TLDR
Evidence is provided of close interaction between PMNL and myocardial EC, resulting in enhanced sLT formation via transcellular biosynthesis, originating from transfer of PMNL-derived LTA4 to EC. Expand
...
1
2
3
...