Different usage of two polyadenylylation signals in transcription of the N-myc gene in rat tumor cells.

@article{Sugiyama1991DifferentUO,
  title={Different usage of two polyadenylylation signals in transcription of the N-myc gene in rat tumor cells.},
  author={Akinori Sugiyama and Yohei Miyagi and Yumi Shirasawa and Yoshiyuki Kuchino},
  journal={Oncogene},
  year={1991},
  volume={6 11},
  pages={2027-32}
}
The complete nucleotide sequence of a rat genomic DNA fragment of 6.9 kbp containing the entire N-myc gene was determined. A unique structural feature of the rat N-myc gene is the presence of two polyadenylation signals in the exon 3 region resulting in formation of two poly(A)+ N-myc mRNAs of 2.9 and 2.2 kb length. Elevated expression of the 2.9 kb mRNA, which was not due to gene amplification, was observed in normal rat tissues such as brain, adrenal and testis, and in rat tumor cells such as… CONTINUE READING