Different role of Apaf-1 in positive selection, negative selection and death by neglect in foetal thymic organ culture.

@article{Matsuki2002DifferentRO,
  title={Different role of Apaf-1 in positive selection, negative selection and death by neglect in foetal thymic organ culture.},
  author={Yasumasa Matsuki and Huang-ge Zhang and Hey Chi Hsu and Ping Hsing Yang and Tao Zhou and Christopher H. Dodd and Francesco Cecconi and P. Gruss and Takushi Tadakuma and John Douglas Mountz},
  journal={Scandinavian journal of immunology},
  year={2002},
  volume={56 2},
  pages={
          174-84
        }
}
Apoptotic protease-activating factor 1 (Apaf-1) is a component of the apoptosome which is required for the activation of procaspase-9. As Apaf-1 knockout (KO) (Apaf-1-/-) mice die before birth, the role of Apaf-1 during thymic selection was investigated using 5 day foetal thymic organ culture (FTOC) of thymi obtained at gestational day 15. There was a lower ratio of CD4 single-positive (SP) to CD8 SP cells and decreased apoptosis of CD4+CD8+ (DP) thymocytes from Apaf-1-/- mice compared with… CONTINUE READING
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