Different ploidy levels of megakaryocytes generated from peripheral or cord blood CD34+ cells are correlated with different levels of platelet release.

@article{Mattia2002DifferentPL,
  title={Different ploidy levels of megakaryocytes generated from peripheral or cord blood CD34+ cells are correlated with different levels of platelet release.},
  author={Gianfranco Mattia and Francesca Vulcano and Luisa Milazzo and Alessandra Barca and Giampiero Macioce and Adele Giampaolo and Hamisa Jane Hassan},
  journal={Blood},
  year={2002},
  volume={99 3},
  pages={888-97}
}
Ploidy could be the key to understanding megakaryocyte (MK) biology and platelet production. Human CD34+ cells purified from umbilical cord blood (CB) and peripheral blood (PB) were investigated on their capability to give rise, in a serum-free medium containing thrombopoietin, to MKs and platelets. CB-MKs showed reduced polyploidization and platelet number compared with PB-MKs, but a similar membrane phenotype. Most CB-MKs showed a 2N content of DNA (approximately 80%) and only 2.6% had 8N… CONTINUE READING