Different pathways of choline metabolism in two choline-independent strains of Streptococcus pneumoniae and their impact on virulence.

@article{Kharat2008DifferentPO,
  title={Different pathways of choline metabolism in two choline-independent strains of Streptococcus pneumoniae and their impact on virulence.},
  author={Arun S. Kharat and Dalia Denapaite and Florian Gehre and Reinhold Br{\"u}ckner and Waldemar Vollmer and Regine Hakenbeck and Alexander Tomasz},
  journal={Journal of bacteriology},
  year={2008},
  volume={190 17},
  pages={5907-14}
}
The two recently characterized Streptococcus pneumoniae strains--R6Chi and R6Cho(-)--that have lost the unique auxotrophic requirement of this bacterial species for choline differ in their mechanisms of choline independence. In strain R6Chi the mechanism is caused by a point mutation in tacF, a gene that is part of the pneumococcal lic2 operon, which is essential for growth and survival of the bacteria. Cultures of lic2 mutants of the encapsulated strain D39Chi growing in choline-containing… CONTINUE READING