• Corpus ID: 31257175

Different mechanisms of cell polarisation in vegetative and shmooing growth in fission yeast.

@article{Niccoli2002DifferentMO,
  title={Different mechanisms of cell polarisation in vegetative and shmooing growth in fission yeast.},
  author={Teresa Niccoli and Paul Nurse},
  journal={Journal of cell science},
  year={2002},
  volume={115 Pt 8},
  pages={
          1651-62
        }
}
Schizosaccharomyces pombe cells have two polarised growth modes: an intrinsic vegetative growth mode, determined by an internal positioning mechanism and an extrinsic shmooing growth mode, activated by external pheromone. We have analysed the role of the cell end marker Tea1p, the CLIP170 like protein Tip1p, the kinesin like protein Tea2p and the Dyrk-like kinase Pom1p, during the switch between the two growth patterns, with the intention of studying the switch away from the vegetative growth… 

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References

SHOWING 1-10 OF 49 REFERENCES

Pom1p, a fission yeast protein kinase that provides positional information for both polarized growth and cytokinesis.

A gene, pom1, is identified that provides positional information for both growth and division in S. pombe and may position the growth and cytokinesis machineries by interaction with both the actin and microtubule cytoskeletons.

Fission yeast Pom1p kinase activity is cell cycle regulated and essential for cellular symmetry during growth and division

It is shown that Pom1p kinase activity is cell cycle regulated in correlation with the state of cellular symmetry: the activity is high during symmetrical growth and division, but lower when cells grow at just one end, and there may be related roles of homologous protein kinases ubiquitously present in all eukaryotes.

Regulation of Cell Polarity by Microtubules in Fission Yeast

A system in which normally cylindrical fission yeast synchronously form branched cells at high frequency upon treatment with the microtubule-depolymerizing drug thiabendazole (TBZ) suggests that microtubules may be of greatest physiological importance for the maintenance of cell shape at specific points in the cell cycle.

Role of small G proteins in yeast cell polarization and wall biosynthesis.

In the vegetative (mitotic) cycle and during sexual conjugation, yeast cells display polarized growth, giving rise to a bud or to a mating projection, respectively, and G proteins can work as molecular switches because of their ability to shift between an active GTP- bound state and an inactive GDP-bound state.

RHO GTPases in the control of cell morphology, cell polarity, and actin localization in fission yeast

The Rho GTPases provide the coordinated regulation of cell wall biosynthetic enzymes and actin organisation required to maintain cell integrity and polarised growth in S. pombe.

Growth in cell length in the fission yeast Schizosaccharomyces pombe.

It is concluded that NETO only happens if: the cell length is greater than a critical value of 9.0-9.5 microns and the cell has traversed the first 0.3-0.35 of the cycle and passed early G2, in which cell size is also reduced.

Fission yeast cell morphogenesis: identification of new genes and analysis of their role during the cell cycle

To identify new genes involved in the control of cell morphogenesis in the fission yeast Schizosaccharomyces pombe we have visually screened for temperature-sensitive mutants that show defects in

Asymmetrically localized Bud8p and Bud9p proteins control yeast cell polarity and development

Bud8p is proposed as a landmark for bud initiation at the distal cell pole, where Bud9p acts as inhibitor, in response to nitrogen starvation, asymmetric localization of Bud9P is averted, favouring Bud8p‐mediated cell division at thedistal pole.

Growth Polarity and Cytokinesis in Fission Yeast: The Role of the Cytoskeleton

The distribution of F-actin in the fission yeast Schizosaccharomyces pombe was investigated by fluorescence microscopy using rhodamine-conjugated phalloidin and a system of cytoplasmic microtubules extending between the cell ends was revealed.